Each siRNA, miRNA mimic or inhibitor (GenePharm, Shanghai, China) was transfected into CAL27 cells for 48 h using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Total RNA was extracted using the Trizol reagent (Invitrogen, Carlsbad, CA), and then reverse transcribed to cDNA using PrimeScriptTM RT-PCR Kit (Takara, Dalian, China) according to the manufacturer’s instructions. For miRNA quantitation, reverse transcription was performed using the PrimeScript RT Reagent Kit (Takara, Dalian, China) with specific stem-loop primers. Quantitative Real-Time PCR (qRT-PCR) was performed using SYBR® Premix DimerEraser™ (Takara, Dalian, China) in a Roche LightCycler 480 II Real-Time PCR system (Roche, Basel, Switzerland). The threshold cycle value (Ct) of each product was determined and normalized against that of the internal control GAPDH or U6 (for miRNA), and the differences were compared by t-test using SPSS version 23.0, the statistical significance set at P < 0.05.
Mirna mimic or inhibitor
Manufactured by GenePharma
Sourced in China
MiRNA mimic or inhibitor is a type of laboratory equipment used in molecular biology research. It is designed to either mimic or inhibit the function of specific microRNAs (miRNAs), which are small, non-coding RNA molecules that play a role in gene expression regulation. The core function of this product is to facilitate the study of miRNA-mediated biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Lightcycler 480 2 real time pcr system, by Roche (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Primescript rt pcr kit, by Takara Bio (1 mentions)
Sybr premix dimereraser, by Takara Bio (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Mir 199b 5p mimic, by GenePharma (1 mentions)
Hek 293t, by GenePharma (1 mentions)
2 protocols using mirna mimic or inhibitor
1
Quantification of miRNA and mRNA Expression
2
Modulation of miR-199b-5p Activity
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HEK293T, human endometrial stromal cell (HESC), and Ishikawa cell lines were obtained from the American Type Culture Collection and cultured in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (Gibco). MiR-199b-5p mimic (double-stranded RNA (dsRNA) oligonucleotides) and miR-199b-5p inhibitor (single-stranded, chemically modified oligonucleotides) (GenePharma) were used for overexpression and inhibition of miR-199b-5p activity in HEK293T and Ishikawa cells (Supplemental Table 2), respectively. To analyze the function of an miRNA, cells were plated in six-well plates and cultured to 50%-70% confluence before transfection with MiR-199b-5p mimic or inhibitor (GenePharma) using Lipofectamine LTX (Invitrogen) according to the manufacturer's instructions. Cells were transfected with RNAs at a final concentration of 10 nM; the negative controls for miRNA mimic or inhibitor (GenePharma) were transfected as matched controls.
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