Western blotting of primary cultures and tissue samples were processed as previously described (Perez-Asensio et al., 2013 (link)). Membranes were then incubated with the following antibodies: phosphorylated STAT1 and STAT3 (all from Cell Signaling Technology, 1:1000); DCX (Cell Signaling Technology 1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:500), Nestin (1:500, either from Merck-Millipore or BD Bioscience San Jose, CA, USA), Musashi (Millipore, 1:500), Mash1 (BD Bioscience, 1:500), total STAT-1 (BD transduction, 1:1000), total STAT-3 (BD Bioscience, 1:1000), Notch-ICD (Abcam, Cambridge, UK; 1:500), Numb (Abcam, 1:500); and Tubulin (Sigma, 1:50000), or Actin (Sigma, 1:50000) as loading controls.
Musashi
Manufactured by Merck Group
Sourced in United States
Musashi is a versatile laboratory equipment designed for various applications in research and analytical settings. Its core function is to provide a controlled and consistent environment for various experimental procedures. The product specifications and features are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Nestin, by Merck Group (2 mentions)
Total stat 1, by BD (1 mentions)
Mash1, by BD (1 mentions)
Total stat 3, by BD (1 mentions)
Protease inhibitor mixture, by Roche (1 mentions)
Hrp conjugated secondary antibody, by Dianova (1 mentions)
Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions)
Na3vo4, by Merck Group (1 mentions)
Enhanced chemiluminescence, by Cytiva (1 mentions)
2 protocols using musashi
1
Western Blotting of Neural Stem Cell Markers
2
Western Blot Analysis of Neural Markers
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Tumor lysates were prepared in RIPA buffer supplemented with a protease inhibitor mixture (Roche) and the phosphatase inhibitors NaF and Na3VO4 (Sigma). Then, 40 µg each of the cell lysates was separated by SDS-PAGE and blotted onto nitrocellulose membranes. The blots were probed with the indicated antibodies and developed by enhanced chemiluminescence (Amersham Biosciences). The following antibodies were used: sex-determining region Y (SRY)-box 2 (Sox2) (R&D Systems), Nestin (Millipore), Musashi (Millipore), GFAP (DAKO), Actin (Santa Cruz Biotechnology), and HRP-conjugated secondary antibodies (Dianova).
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