Rpmi 1640 l glutamine

To test the biological activity of the products, Sabouraud dextrose broth (SDB) and Sabouraud dextrose agar (SDA) were purchased from Difco Laboratories (Detroit, MI, USA) and agar-cornmeal from HiMédia Laboratories (Mumbai, India), and RPMI-1640-L-glutamine (without sodium bicarbonate) (Sigma-Aldrich, São Paulo, SP, Brazil) culture media were used. They were prepared and used according to the manufacturers' instructions.

The test substance α-pinene, the antifungals (amphotericin B; caspofungin), ergosterol, and dimethylsulfoxide (DMSO) were commercially obtained from the manufacturer Sigma Aldrich^®. Sorbitol was obtained from VETEC Química Fina Ltd.a-Rio de Janeiro/RJ. The culture medium Sabouraud Dextrose Agar (SDA) (Difco Laboratories, Detroit, MI, USA) was used for the maintenance of the Candida strains. The liquid culture medium Roswell Park Memorial Institute (RPMI)-1640-L-glutamine (without sodium bicarbonate) (Sigma-Aldrich^®, São Paulo, SP, Brazil) was used for the antifungal assays. Both culture media were prepared according to the manufacturer’s instructions. The α-pinene was dis-solved in DMSO (with a final concentration of 0.13% and 1% for the cytotoxicity Aasay and anti-fungal activity, respectively).