Insulin (Crystal Chem), total GLP-1 (MesoScale Discovery), and acetaminophen (Sekisui Diagnostics) were measured during experiments shown in Fig. 3 . Insufficient amount of blood was obtained from one Control VSG for Insulin, total GLP-1 and acetaminophen levels at 15 min. Postprandial plasma obtained at termination of studies (see above for details) was used to measure IGF-1 (R&D Systems), Activin-A (R&D Systems), Myostatin (GDF8) (R&D Systems), FGF21 (R&D Systems), L-amino acids (Abcam), Erythropoietin (Abcam), FGF23 (Abcam), Ferritin (Abcam), Hemoglobin (Abcam), Phosphate (Abcam), 25(OH) Vitamin D (Abcam). Iron levels in cecal contents were measured with Iron Assay Kit (Abcam). Glycogen content was measured in liver and muscle (tibialis anterior) samples with Glycogen Assay kit (Sigma-Aldrich). All sampled blood was collected via tail vein in EDTA-coated tubes. All assays were performed according to the manufacturer’s instructions.
Hemoglobin
Manufactured by Abcam
Hemoglobin is a protein found in red blood cells that is responsible for carrying oxygen from the lungs to the body's tissues and carbon dioxide from the tissues back to the lungs.
Automatically generated - may contain errors
Lab products found in correlation
Acetaminophen, by Sekisui (2 mentions)
Ferritin, by Abcam (1 mentions)
Iron assay kit, by Abcam (1 mentions)
Fgf21, by R&D Systems (1 mentions)
Erythropoietin, by Abcam (1 mentions)
Igf 1, by R&D Systems (1 mentions)
Total glp 1, by Mesoscale (1 mentions)
Fgf23, by Abcam (1 mentions)
Glycogen assay kit, by Merck Group (1 mentions)
Activin a, by R&D Systems (1 mentions)
Insulin, by Crystal Chem (1 mentions)
Osteopontin, by Abcam (1 mentions)
Osteopontin, by Santa Cruz Biotechnology (1 mentions)
Hoechst solution, by Thermo Fisher Scientific (1 mentions)
Osterix, by Abcam (1 mentions)
Ter 119, by R&D Systems (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
2 protocols using hemoglobin
1
Comprehensive Metabolic Profiling in VSG
2
Characterization of Vascular Calcification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded human and mouse vascular tissues were stained using Masson Goldner trichrome to detect erythrocytes (bright red), connective tissue (light green), and bone structures (green). Calcification (red signal) was assessed using Alizarin red and van Kossa staining or 5-bromo-4-chloro-3indolyl phosphate/nitro blue tetrazolium as a substrate for alkaline phosphatase. Perl iron stain was used to detect tissue iron. Immunohistochemical analysis was performed using antibodies against eNOS (Novus Biologicals), glycophorin A (CD235a; Dako), hemoglobin (Abcam), methemoglobin (LifeSpan Biosciences), osteopontin (Abcam), osterix (Abcam), smooth muscle actin (Sigma), or TER-119 (R&D Systems) and examined under an Olympus BX51 microscope using image analysis software (Image-Pro Plus; Media Cybernetics).
Immunofluorescence and confocal microscopy analyses were performed on cryo-embedded tissue sections using primary antibodies against CD235a, osteopontin, or eNOS (Santa Cruz Biotechnology) followed by fluorescence-labeled donkey anti-mouse or donkey anti-rabbit secondary antibodies (Life Technologies) and Hoechst solution (Thermo Scientific).
Sections were examined under an Andor Revolution XD spinning disc confocal Olympus IX81 microscope. Images were analyzed using ImageJ 1.50i software.
Immunofluorescence and confocal microscopy analyses were performed on cryo-embedded tissue sections using primary antibodies against CD235a, osteopontin, or eNOS (Santa Cruz Biotechnology) followed by fluorescence-labeled donkey anti-mouse or donkey anti-rabbit secondary antibodies (Life Technologies) and Hoechst solution (Thermo Scientific).
Sections were examined under an Andor Revolution XD spinning disc confocal Olympus IX81 microscope. Images were analyzed using ImageJ 1.50i software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!