Total protein was extracted from FC tissue and HMC3 cells using PMSF and RIPA buffer and quantified with the bicinchoninic acid method. The western V3 workflow (Bio-Rad) was used for western blot analysis. The primary antibodies [anti-GAP-43 (ab12274 and ab11136) and anti-GAPDH (ab181602)] and secondary antibody [horseradish peroxidase-conjugated goat anti-rabbit IgG H&L (ab6721)] were purchased from Abcam (Shanghai, China).
Horseradish peroxidase conjugated goat anti rabbit igg h l ab6721
Manufactured by Abcam
Sourced in China
Horseradish peroxidase-conjugated goat anti-rabbit IgG H&L (ab6721) is a secondary antibody that recognizes the heavy and light chains of rabbit immunoglobulin G (IgG). It is conjugated with horseradish peroxidase, an enzyme commonly used as a reporter molecule in various immunoassays and detection methods.
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Lab products found in correlation
2 protocols using horseradish peroxidase conjugated goat anti rabbit igg h l ab6721
1
Quantifying Protein Expression in Cells
2
Quantification of Neuronal GAP-43 Protein
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Total protein was extracted from FC tissue and HMC3 cells using PMSF and RIPA buffer and quanti ed with the bicinchoninic acid method. The western V3 work ow (Bio-Rad) was used for western blot analysis. The primary antibodies [anti-GAP-43 (ab12274 and ab11136) and anti-GAPDH (ab181602)] and secondary antibody [horseradish peroxidase-conjugated goat anti-rabbit IgG H&L (ab6721)] were purchased from Abcam (Shanghai, China).
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