Microarray analysis was conducted as a previous report [18 (link)]. In brief, total RNA from 5 pairs of CC and paracancerous tissues was extracted. Next, cDNA was synthesized using 0.5 μg total RNA via a GeneChip 3’In-vitro Transcription Express Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA, 902789). Then the cDNA was fragmented and hybridized with human LncRNA Array V3.0 (Arraystar Inc., USA, AS-LNC-H-V4.0). After that, the chips were washed and scanned using a GeneChip™ Scanner 3000 7G system (Thermo Fisher, 000213).
Genechip 3 in vitro transcription express kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The GeneChip 3'In-vitro Transcription Express Kit is a laboratory equipment product used for the in vitro transcription of polyadenylated RNA from DNA templates. The kit contains the necessary reagents and components to perform this RNA synthesis process.
Automatically generated - may contain errors
3 protocols using genechip 3 in vitro transcription express kit
1
Microarray Analysis of lncRNA Expression
2
Profiling lncRNA Expression in CRC Serum EVs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microarray analysis was performed following the steps described previously in the literature [16 (link)]. In brief, total RNA was extracted from the serum EVs from 6 CRC patients and 6 healthy subjects. Subsequently, total RNA from the EVs was extracted and 0.5 μg RNA was used for cDNA synthesis using a GeneChip 3′. In vitro transcription express kit (902789, Thermo Fisher Scientific, Waltham, MA, USA). Subsequently, the cDNA was segmented and hybridized with a human lncRNA expression array V3.0 (AS-LNC-H-V4.0, Arraystar Inc. Rockville, MD, USA). After hybridization, the microarray was washed and scanned with a GeneChip Scanner 3000 7G System (000213, Thermo Fisher Scientific).
3
Differential lncRNA Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microarray analysis was performed to screen out the differentially expressed lncRNAs between tumor and normal tissues. In brief, 0.5 µg total RNA from either tumor tissues or normal tissues was reversely transcribed to cDNA using a GeneChip 3'In vitro Transcription Express Kit (Thermo Fisher Scientific, Waltham, MA, USA). Then the cDNA was hybridized with Human LncRNA Expression Array V4.0 (Arraystar, Rockville, MD, USA), and then the chip was washed and scanned on a GeneChipTM Scanner3000 7G System (Thermo Fisher).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!