Amicon ultra 0.5 regenerated cellulose filters

Manufactured by Merck Group

Sourced in Italy

The Amicon Ultra-0.5 regenerated cellulose filters are laboratory equipment designed for sample preparation and concentration. They are used to concentrate and desalt samples through ultrafiltration. The filters have a regenerated cellulose membrane and are available in different molecular weight cut-off sizes.

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Lab products found in correlation

Taq dna polymerase, by Takara Bio (1 mentions)

Close spelling variants of this product

Amicon Ultra (740 citations) Amicon filters (181 citations) Amicon Ultra filters (132 citations) Amicon Ultra-0.5 (105 citations) Cellulose filters (15 citations) Amicon Ultra-0.5 filters (14 citations) Regenerated cellulose (11 citations) Amicon Ultra 0.5 mL Centrifugal Filters Ultracel 50 K Regenerated Cellulose 50000 NMWL (2 citations)

3 protocols using amicon ultra 0.5 regenerated cellulose filters

In Vitro Digestion and Antioxidant Analysis

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Chemicals and reagents for in vitro digestion, enzymatic assays, and antioxidant activity analysis were from Sigma-Aldrich (Milan, Italy), whereas the solvents for mass spectrometry analysis were supplied by Biorad (Hercules, CA, USA). Medium M17 for streptococci growth and lactose were supplied by Oxoid (Basingstoke, Hampshire, UK). Whey protein concentrate (WPC80) containing 81% (w/w) protein and 4.5% (w/w) fat was purchased from a local producer (Reire srl, Reggio Emilia, Italy).
The ultra-filtration units (Amicon Ultra-0.5 regenerated cellulose filters; cut-off of 10 kDa) were purchased from Millipore (Milan, Italy). Standard peptides for quantitative analysis (APFPE, IPP and VPP; 99% purity) were synthesized by Bio-Fab Research (Rome, Italy).

Helal A., Pierri S., Tagliazucchi D, & Solieri L. (2023). Effect of Fermentation with Streptococcus thermophilus Strains on In Vitro Gastro-Intestinal Digestion of Whey Protein Concentrates. Microorganisms, 11(7), 1742.

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Milk Peptides Characterization

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All MS/MS reagents were from Biorad (Hercules CA, U.S.A.), whereas the remaining chemicals were purchased from Sigma-Aldrich (Milan, Italy) unless otherwise stated. De Man, Rogosa and Sharpe (MRS) medium was provided by Oxoid (Basingstoke, Hampshire, England). Amicon Ultra-0.5 regenerated cellulose filters with a molecular weight (MW) cut-off of 3 kDa were supplied by Millipore (Milan, Italy). Ultra-high-temperature-treated (UHT) skimmed bovine milk was obtained from a local producer (protein, 3.15%; fat, 0.3%; lactose, 4.95%). VPP and IPP peptides (95% purity) were synthesized by DBA (Milan, Italy). The absorbance was read using a Jasco V-550 UV/Vis spectrophotometer (Orlando FL, U.S.A.), with the exception of DNA samples that were quantified by Nanodrop ND-1000 Spectrophotometer (Wilmington, DE, USA). Taq DNA polymerase was from Takara (Kyoto, Japan), while primers were provided by MWG (Heidelberg, Germany).

Solieri L., Rutella G.S, & Tagliazucchi D. (2015). Impact of non-starter lactobacilli on release of peptides with angiotensin-converting enzyme inhibitory and antioxidant activities during bovine milk fermentation. Food microbiology, 51.

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Angiotensin-Converting Enzyme Inhibitory Peptides

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All MS/MS reagents were obtained from Biorad (Hercules, CA, U.S.A.), whereas the chemicals and enzymes for the digestion procedure, ACE assay and degree of hydrolysis determination were purchased from Sigma-Aldrich (Milan, Italy). Amicon Ultra-0.5 regenerated cellulose filters with a molecular weight (MW) cut-off of 3 kDa were supplied by Millipore (Milan, Italy). The whole goat milk was obtained from farms at El-Alamin and Sidi-Barani areas around Alexandria (Egypt).
Valine-proline-proline (VPP) and isoleucine-proline-proline (IPP) peptides (95% purity) were synthesized by DBA (Milan, Italy). All the other reagents were from Carlo Erba (Milan, Italy).

Tagliazucchi D., Shamsia S., Helal A, & Conte A. (2017). Angiotensin-converting enzyme inhibitory peptides from goats' milk released by in vitro gastro-intestinal digestion. International Dairy Journal., 71.

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