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Lumipulse g system

Manufactured by Fujirebio
Sourced in Spain, Belgium, Japan

The Lumipulse G System is an automated immunoassay analyzer designed for in vitro diagnostic testing. It utilizes chemiluminescent enzyme immunoassay (CLEIA) technology to quantitatively measure various analytes in biological samples.

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4 protocols using lumipulse g system

1

Serum Biomarkers YKL-40 and KL-6 Measurement

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YKL-40 levels were measured in serum samples obtained at each visit by an YKL-40 enzyme immunoassay kit (Quidel, San Diego, CA, USA), in accordance with the manufacturer’s instructions.
KL-6 levels were measured in serum samples obtained at each visit by a chemiluminescent enzyme immunoassay (Lumipulse G KL-6, Fujirebio Iberia SL, Barcelona, Spain) using the Lumipulse G System and in accordance with the manufacturer’s instructions.
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2

Longitudinal Assessment of HBV and HIV Biomarkers

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Serum HBV-DNA viral load was quantified at baseline and every 6–12 months by a commercial polymerase chain reaction (PCR)-based assay (COBAS AmpliPrep/COBAS TaqMan, detection limit 12 IU/mL; or COBAS Amplicor HBV Monitor, detection limit 60 IU/mL [Roche Diagnostics Systems, Meylan, France]). Serum HIV-1 RNA viral load was measured at baseline and every 6–12 months using either a branched DNA technique (bDNA Quantiplex 3.0, detection limit 50 copies/mL [Bayer Diagnostics, Cergy-Pontoise, France]) or real-time PCR technique (COBAS AmpliPrep/COBAS TaqMan HIV-1 test, detection limit 40 copies/mL [Roche Molecular Systems, Meylan, France]). Qualitative HBsAg, HBeAg, and antibodies were detected at cohort inclusion and every yearly visit using commercial enzyme immunoassays (EIA).
Serum qHBcrAg (U/mL) was measured at baseline and every 6 months using commercially available, automated HBcrAg chemiluminescence EIA (Lumipulse G System; FujiRebio Europe, Gent, Belgium) [22 (link)]. For levels of qHBcrAg >7 log, a dilution 1/100 was performed as recommended by the manufacturer. In addition, anti-HBc antibodies (both immunoglobulin [Ig]G and IgM) were quantified at baseline and every 6–12 months using ARCHITECT Anti-HBc II assay (Abbott Laboratories, Rungis, France), with an automated ARCHITECT i4000 system.
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3

Detecting SARS-CoV-2 in Breastmilk

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Breastmilk samples were centrifuged (twice for 5 minutes each at 2000g), and approximately 200 μL of the resulting supernatant was used for detection and quantitation of SARS-CoV-2 nucleocapsid protein antigens via a chemiluminescent enzyme immunoassay (Lumipulse G SARS-CoV-2 Ag assay with the LUMIPULSE G System; Fujirebio). To avoid false-negative results, an internal control (10 μL) obtained from breastmilk samples of 7 control mothers who were breastfeeding was resuspended with supernatant (1 mL). Antigenic tests were also performed using these samples.
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4

Lumipulse C-Peptide Immunoassay Protocol

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Lumipulse® C-Peptide immunoassay (Fujirebio, Tokyo, Japan) for the quantitative determination of C-peptide in serum or plasma specimens, is based on CLEIA technology by a twostep sandwich immunoassay method on the fully automated Lumipulse® G System (Fujirebio, Tokyo, Japan). The Lumipulse® C-Peptide uses 30 µL of specimen for each assay. As reported by the manufacturer, the observed range of C-peptide values in apparently healthy subjects was 0.71 -2.58 ng/mL. The precision of Lumipulse® C-Peptide resulted ≤5.1%; Lower Limit of Detection (LLoD) and Quantitation (LLoQ) corresponded to 0.0109 ng/mL. Dilution testing reported that linearity was found in a range of 0.57 -30.00 ng/mL.
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