Norfloxacin

Manufactured by Bioanalyse

Norfloxacin is a synthetic antibacterial agent that belongs to the fluoroquinolone class of antimicrobial drugs. It is used as a laboratory standard for the analysis and testing of various biological samples.

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2 protocols using norfloxacin

Antimicrobial Susceptibility Testing of ESBL-Producing K. pneumoniae

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The ESBL-producing K. pneumoniae isolates were tested for
antimicrobial susceptibility testing (AST) using disk diffusion technique
according to the Clinical and Laboratory Standards Institute (CLSI)
instructions⁹. Antimicrobials were classified as follows: aztreonam (ATM, 30 μg),
gentamicin (CN, 10 μg), ciprofloxacin (CIP, 5 μg), ceftazidime (CAZ, 30 μg),
levofloxacin (LEV, 5 μg), amoxicillin/clavulanate (AMC, 30 μg), trimethoprim
(TMP, 5 μg), norfloxacin (NOR, 10 μg), cefotaxime (CTX, 30 μg), nitrofurantoin
(F, 300 μg), imipenem (IPM, 10 μg), chloramphenicol (C, 30 μg), tetracycline
(TE, 30 μg), and ceftriaxone (CRO, 30 μg) (Bioanalyse, Turkey). MDR isolates
were determined according to the previous definition (resistance to at least one
member of three antibiotics classes)¹⁰. Escherichia coli ATCC 25922 and K.
pneumoniae ATCC 700603 were used as quality control strains.

Raouf F.E., Benyagoub E., Alkhudhairy M.K., Akrami S, & Saki M. (2022). Extended-spectrum beta-lactamases among Klebsiella pneumoniae from Iraqi patients with community-acquired pneumonia. Revista da Associação Médica Brasileira, 68(6), 833-837.

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Antibiotic Sensitivity Testing by Agar Disc Diffusion

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Antibiotic sensitivity testing (AST) was performed by Kirby-Bauer test using Agar Disc Diffusion Method in Mueller-Hinton medium, following recommendations of the Clinical Laboratory Standards Institute (CLSI, 2018, 28 th ) for Antibiotic sensitivity testing in the medical laboratories. The following 7 antibiotics of fluoroquinolones were used: Nalidixic Acid (30µg), Cipr Ofloxacin (10µg), Norfloxacin (10µg), Ofloxacin (5µg), Lomafloxacin (10µg), LevOfloxacin (5µg), and Trovafloxacin (10µg), which were all obtained from BioAnalyse, Turkey [14] .

Bashi M.A., & Salman E.D. (2020). Bacteriological and Molecular Study of Fluoroquinolones Resistance in Pseudomonas aeruginosa Isolated From Different Clinical Sources. Iraqi Journal of Science.

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