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Stemi 11

Manufactured by Zeiss
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Sourced in Germany

The Stemi 11 is a stereo microscope designed for basic observation and inspection tasks. It provides a magnification range of 6.4x to 50x, allowing users to view specimens in detail. The Stemi 11 features a sturdy, ergonomic design and is equipped with LED illumination for clear, uniform lighting.

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Lab products found in correlation

Alexa 568, by Thermo Fisher Scientific (1 mentions) Lsm 510, by Zeiss (1 mentions) Xc10 camera, by Olympus (1 mentions) Bx51 microscope, by Olympus (1 mentions) Axio scan z1 microscope, by Zeiss (1 mentions)

Spelling variants of this product

Stemi SV 11 Apo (10 citations) Stemi SV 11 Stereoscope (2 citations) Zeiss Stemi SV 11 Apo Stereo Microscope (2 citations) Stemi SV 11 Apo stereoscope (2 citations)

4 protocols using stemi 11

1

Pathological Analysis of Murine Tissues

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Macroscopic pathological analysis of murine tissues including ECC and PDAC was performed and white-light and fluorescence pictures were taken by using a Zeiss Stemi 11 fluorescence stereomicroscope (Zeiss, Oberkochen, Germany).
Falcomatà C., Bärthel S., Ulrich A., Diersch S., Veltkamp C., Rad L., Boniolo F., Solar M., Steiger K., Seidler B., Zukowska M., Madej J., Wang M., Öllinger R., Maresch R., Barenboim M., Eser S., Tschurtschenthaler M., Mehrabi A., Roessler S., Goeppert B., Kind A., Schnieke A., Robles M.S., Bradley A., Schmid R.M., Schmidt-Supprian M., Reichert M., Weichert W., Sansom O.J., Morton J.P., Rad R., Schneider G, & Saur D. (2021). Genetic Screens Identify a Context-Specific PI3K/p27Kip1 Node Driving Extrahepatic Biliary Cancer. Cancer Discovery, 11(12), 3158-3177.
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2

Quantification of Metastases in Mouse PDAC

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Macroscopic analysis of mouse tissues, PDAC and metastases was performed using a Zeiss Stemi 11 fluorescence stereomicroscope. At necropsy, all abdominal organs and the lungs were investigated macroscopically for metastases as described17 (link),43 (link). For microscopic quantification of metastases at least three series of sections (100 μm between the different series) of paraffin embedded lungs and livers of 20 mice per genotype with PDAC were prepared, H&E stained and investigated for the presence of metastases by an examiner blinded to the genotype of the animals.
Schönhuber N., Seidler B., Schuck K., Veltkamp C., Schachtler C., Zukowska M., Eser S., Feyerabend T.B., Paul M.C., Eser P., Klein S., Lowy A.M., Banerjee R., Yang F., Lee C.L., Moding E.J., Kirsch D.G., Scheideler A., Alessi D.R., Varela I., Bradley A., Kind A., Schnieke A.E., Rodewald H.R., Rad R., Schmid R.M., Schneider G, & Saur D. (2014). A next-generation dual-recombinase system for time and host specific targeting of pancreatic cancer. Nature medicine, 20(11), 1340-1347.
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3

Histological Analysis of PFA-fixed Specimens

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PFA-fixed specimens were used for serial sectioning. They were dehydrated and embedded in paraffin. Serial sectioning was performed using a rotary microtome (Microm, HM 355 S). Serial sections of 7 μm thickness were collected on microscope slides and stained according to Heidenhain's Azan technique (Heidenhain, 1915) . Images were taken with an XC10 Olympus camera mounted on an Olympus BX51 microscope operated with dotSlide software. The clearing-andstaining procedure followed the protocol provided by Dingerkus and Uhler (1977) with the exception, that no alizarin red was used due to the absence of bones. Cleared-and-stained specimens were examined with a Zeiss Stemi 11 and images were taken by an attached camera (ColorView) operated by AnalySIS software. Specimens fixed with Dent's fixative were used for whole mount antibody staining. A monoclonal antibody against collagen II (116B3-collagen II, obtained from the Developmental Studies Hybridoma Bank) and Alexa 568 (Thermo Fischer Scientific) as fluorescent secondary antibody were used to specifically stain cartilages. Image stacks (10 μm z-plane, 1 AU) were produced using a confocal laser scanning microscope (LSM 510, Zeiss).
Lukas P, & Olsson L. (2020). Sequence of chondrocranial development in the oriental fire bellied toad Bombina orientalis. Journal of morphology, 281(6).
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4

Detailed Histological Staining Protocol

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The PFA-fixed specimens were dehydrated, embedded in paraffin, and serial sectioned at 7 μm thickness using a rotary microtome (Microm, HM 355 S). The sections were stained according to Heidenhain's Azan technique [59 ]. Images were taken with an Hitachi HV-F202SCL camera mounted on an Zeiss AxioScan Z1 microscope operated with Zen 3.1 software. The clearing-and-staining procedure followed the protocol by Dingerkus and Uhler [60 ] with the exception that no alizarin red was used due to the absence of bones. Cleared-and-stained specimens were examined with a Zeiss Stemi 11 and images were taken by an attached camera (ColorView) operated by AnalySIS software.
Lukas P, & Ziermann J.M. (2022). Sequence of chondrocranial development in basal anurans—Let’s make a cranium. Frontiers in Zoology, 19, 17.
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