The activated partial thromboplastin time was measured using Dade Actin FSL–activated PTT reagent (Siemens Healthcare Diagnostics, Marburg, Germany), prothrombin time (PT) was measured using Dade Innovin reagent (Siemens Healthcare Diagnostics), fibrinogen levels were determined with Multifibren U reagent (Siemens Healthcare Diagnostics), and D-dimer was measured using INNOVANCE D-Dimer kits (Siemens Healthcare Diagnostics). All assays were performed according to the manufacturer instruction using a BCS system (Siemens Healthineers, Erlangen, Germany). Prothrombin fragment 1+2 was measured using Enzygnost F1+2 ELISA kits (Siemens Healthineers) following the manufacturer instructions. Activated coagulation factors in complex with their natural inhibitors (ie, activated FVII:antithrombin [FVIIa:AT], FXIIa:AT, FXIIa:C1 esterase inhibitor [FXIIa:C1Inh], FXIa:AT, FXIa:alpha1-antitrypsin [α1AT], FXIa:C1Inh, FIXa:AT, FXa:AT, and thrombin:antithrombin [T:AT]) were quantified as described.10 (link), 11 (link), 12 (link) In short, FVIIa:AT represents activation of the extrinsic pathway, FXIIa:AT, FXIIa:C1Inh, FXIa:AT, FXIa:α1AT, FXIa:C1Inh, and FIXa:AT represent activation of the intrinsic pathway, and FXa:AT as well as T:AT represent activation of the common pathway of coagulation.
Bcs system
Manufactured by Siemens
Sourced in Germany
The BCS System is a laboratory equipment product manufactured by Siemens. It is designed to perform basic control and automation functions in a laboratory setting. The core function of the BCS System is to monitor and regulate various parameters within the laboratory environment.
Automatically generated - may contain errors
3 protocols using bcs system
1
Comprehensive Coagulation Pathway Analysis
2
Biomarker Assessment in Fasted Subjects
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Blood samples were obtained from each subject early in the morning, following a 10-hour overnight fast by venipuncture from the antecubital vein. The samples were stored in sterile blood collection tubes in refrigerated conditions (4° to 8°C) for no longer than 4 hours during the morning of collection and then sent to an analytical laboratory for testing according to standardized procedures, as follow: hs-C-Reactive Protein, latex enhanced immunoturbidimetric assay (Siemens Advia 1600/1800 Erlangen, Germany); HDL-Cholesterol, Precipitation of the Apolipoprotein B containing lipoproteins with dextran-magnesium-chloride (Siemens Advia 1600/1800 Erlangen, Germany); Glucose, Hexokinase method (Siemens Advia 1600/1800 Erlangen, Germany); Insulin, Chemiluminescence immunoassay (Siemens ACS Centaur System, Erlangen, Germany); Fibrinogen, Clauss method (Siemens BCS System, Erlangen, Germany); Complement factors C3 and C4, Immunoturbidimetric assay (Siemens Advia 1600/1800, Erlangen, German; Total cholesterol (TC) CHOD-POD enzymatic method (Siemens Advia 1600/1800); Triglycerides, enzyme glycerol phosphate oxidase method (GPO) (Siemens Advia 1600/1800 Erlangen, Germany). Leptin CRP, C3 and C4 were determined in serum and fibrinogen was determined in plasma. All assays were performed in duplicate according to the manufacturers' instructions.
3
Biomarker Profiling of Metabolic Health
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Blood samples were obtained from each subject early in the morning, following a 10-hour overnight fast by venipuncture from the antecubital vein. The samples were stored in sterile blood collection tubes in refrigerated conditions (4 to 8 C) for no longer than 4 hours during the morning of collection and then sent to an analytical laboratory for testing according to standardized procedures, as follows: glucose, Hexokinase method (Siemens Advia 1600/1800 Erlangen, Germany); insulin, chemiluminescence immunoassay (Siemens ACS Centaur System, Erlangen, Germany); hs-C-Reactive Protein, latex enhanced immunoturbidimetric assay (Siemens Advia 1600/1800 Erlangen, Germany); Fibrinogen, Clauss method (Siemens BCS System, Erlangen, Germany); Complement factors C3 and C4, Immunoturbidimetric assay (Siemens Advia 1600/1800, Erlangen, German. Leptin, hs-C-Reactive Protein, C3, and C4 were determined in serum and fibrinogen was determined in plasma. All assays were performed in duplicate according to the manufacturers' instructions.
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