α sma

Manufactured by PerkinElmer

α-SMA is a protein marker used in research applications to detect the presence and distribution of smooth muscle cells in various tissues. It is a component of the contractile apparatus and is commonly used as a marker for the identification and analysis of smooth muscle cells.

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Lab products found in correlation

Catalog 4904, by Cell Signaling Technology (1 mentions) Vectra multispectral slide analysis system, by PerkinElmer (1 mentions) Interleukin 6 (il 6), by Abcam (1 mentions) Ab28364, by Abcam (1 mentions) A5228, by Merck Group (1 mentions) Volocity software, by PerkinElmer (1 mentions)

2 protocols using α sma

Immunohistochemical Analysis of Pancreatic Tumors

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Tumor tissue was prospectively obtained at surgery under an Institutional review board-approved protocol following informed consent and de-identified. Formalin fixed pancreatic tissue from human tissue specimens and in vivo experiments in mice were subjected to IHC analysis following staining with Ab against IL-6 (Abcam and eBioscience), pSTAT3 (Catalog 4904; Cell Signaling), α-SMA (Catalog M0851; Dako), PD-L1 (Catalog NBP1-76769) and CD3 (Catalog A0452; Dako). For α-SMA quantification, 20× magnification images of pancreata (8–10 images per mouse) were captured using PerkinElmer’s Vectra multispectral slide analysis system. inForm software tools were used to quantify positive SMA cells (Fast Red chromogen) within each image (Supplementary Figure 1). For analysis of CD3⁺ cells, blinded histological analysis of staining in the pancreas was counted at 40× magnification, with at least 10 fields counted per mouse.

Mace T.A., Shakya R., Pitarresi J.R., Swanson B., McQuinn C.W., Loftus S., Nordquist E., Cruz-Monserrate Z., Yu L., Young G., Zhong X., Zimmers T.A., Ostrowski M.C., Ludwig T., Bloomston M., Bekaii-Saab T, & Lesinski G.B. (2016). IL-6 and PD-L1 antibody blockade combination therapy reduces tumor progression in murine models of pancreatic cancer. Gut, 67(2), 320-332.

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Tumor Vascularity and Pericyte Coverage

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Tumor vascularity and pericyte coverage of the vasculature were determined as previously described [28] (link). Briefly, tumor sections of MMTV-Neu;Mmp9^+/+ (n = 8), C3(1)-Tag;Mmp9^+/+ (n = 8), and C3(1)-Tag;Mmp9^−/− (n = 8) mice were double immunofluorescence stained with anti-CD31 (1:25 dilution, ab28364; Abcam) and α smooth muscle actin (αSMA; 1:1500 dilution, A5228; Sigma-Aldrich, St Louis, MO). CD31-positive (endothelial cell marker) and αSMA-positive (pericyte marker) areas were defined as those with a fluorescence intensity above 150% of the mean of background intensity levels, as determined by Volocity software (PerkinElmer, Waltham, MA). Pericyte coverage was calculated by dividing the αSMA-positive (pericyte) area by the CD31-positive (endothelial cell) area. All images were analyzed without knowledge of mouse genotype.

Park J.H., Rasch M.G., Qiu J., Lund I.K, & Egeblad M. (2015). Presence of Insulin-Like Growth Factor Binding Proteins Correlates With Tumor-Promoting Effects of Matrix Metalloproteinase 9 in Breast Cancer. Neoplasia (New York, N.Y.), 17(5), 421-433.

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