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Sc 8977

Manufactured by BD
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The Sc-8977 is a laboratory centrifuge device. It is designed to separate and isolate different components within liquid samples through the application of centrifugal force. The device's core function is to enable efficient sample preparation and processing for various applications in research and diagnostic laboratories.

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Lab products found in correlation

Ab13970, by Cell Signaling Technology (2 mentions) Fluorescence conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Af1924, by R&D Systems (2 mentions) Anti oct4, by BD (2 mentions)

2 protocols using sc 8977

1

Immunofluorescence Staining of Adherent Cells and Embryoid Bodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adherent cells were cultured on ibidi μ-Slide and fixed in 4% PFA for 30 minutes at 4°C. Embryoid bodies were fixed in 4% PFA for 2h at 4°C and embedded in OCT compound for frozen sections. The samples were incubated with primary antibodies for overnight at 4°C and subsequently with fluorescence-conjugated secondary antibodies (Thermo Fisher Scientific) and DAPI for 1h at RT. The primary antibodies used were: anti-GFP (abcam ab13970, 1:1000), anti-PRDM1 (Cell Signaling Technology 9115, 1:200), anti-SOX17 (R&D AF1924, 1:500), anti-TFAP2C (Santa Cruz Biotechnology sc-8977, 1:200), and anti-OCT4 (BD Biosciences 611203, 1:500). Samples were imaged under Leica SP8 upright or inverted scanning confocal microscope and analysed using Volocity (6.3).
Tang W.W., Castillo-Venzor A., Gruhn W.H., Kobayashi T., Penfold C.A., Morgan M.D., Sun D., Irie N, & Azim Surani M. (2022). Sequential enhancer state remodelling defines human germline competence and specification. Nature cell biology, 24(4), 448-460.
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2

Immunofluorescence Staining of Adherent Cells and Embryoid Bodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adherent cells were cultured on ibidi μ-Slide and fixed in 4% PFA for 30 minutes at 4°C. Embryoid bodies were fixed in 4% PFA for 2h at 4°C and embedded in OCT compound for frozen sections. The samples were incubated with primary antibodies for overnight at 4°C and subsequently with fluorescence-conjugated secondary antibodies (Thermo Fisher Scientific) and DAPI for 1h at RT. The primary antibodies used were: anti-GFP (abcam ab13970, 1:1000), anti-PRDM1 (Cell Signaling Technology 9115, 1:200), anti-SOX17 (R&D AF1924, 1:500), anti-TFAP2C (Santa Cruz Biotechnology sc-8977, 1:200), and anti-OCT4 (BD Biosciences 611203, 1:500). Samples were imaged under Leica SP8 upright or inverted scanning confocal microscope and analysed using Volocity (6.3).
Tang W.W., Castillo-Venzor A., Gruhn W.H., Kobayashi T., Penfold C.A., Morgan M.D., Sun D., Irie N, & Azim Surani M. (2022). Sequential enhancer state remodelling defines human germline competence and specification. Nature cell biology, 24(4), 448-460.
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