Anti phospho jnk thr182 tyr185 antibody

Kahweol was purchased from LKT Laboratories Inc (St Paul, MN, USA). A 40 mM solution of Kahweol was initially prepared in DMSO, stored as small aliquots at −20°C, and thawed and diluted in cell culture medium, as required. Thioacetamide (TAA) was purchased from Sigma Aldrich (St. Louis, MO, USA) and recombinant human TGF-β, (5 ng/mL) was purchased from R&D Systems (Minneapolis, MN, USA). The anti-CTGF antibody and anti-phospho-Smad2/3 antibodies were purchased from Santa Cruz (Dallas, TX, USA). Anti-Collagen antibody was purchased from Abcam (Cambridge, UK) and anti-αSMA antibody was purchased from Sigma Aldrich. Anti-GAPDH antibody, anti-phospho-Smad3 (Ser423/425) antibody, anti-phospho-ERK (Th202/Tyr204) antibody, anti-phospho-JNK (Thr182/Tyr185) antibody and anti-phospho-STAT3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA, USA).

Kahweol was purchased from LKT Laboratories Inc. (St. Paul, MN, USA), lipopolysaccharide (LPS, Escherichia coli 055; B5) was purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-phospho-NFκB antibody, anti-phospho-ERK (Th202/Tyr204) antibody, anti-phospho-JNK (Thr182/Tyr185) antibody, anti-phospho-STAT3 (Tyr705) and anti-GAPDH antibody were purchased from Cell Signaling Technology (Beverly, MA, USA).