Monoclonal anti il 22 clone 8e11

For C. rodentium infection a single colony of strain DBS100 (ATCC 51459; American Type Culture Collection) was transferred to Luria–Bertani (LB) broth and grown to log phase followed by centrifugation and resuspension in PBS. Mice were orally gavaged with 200μl of PBS containing 2x10⁹C. rodentium. To determine bacterial load, intestinal tissue pieces or faecal pellets were weighed and homogenized in sterile PBS and serial dilutions were plated onto Brilliance E. coli/coliform Selective Agar (Fisher Scientific) or LB agar plates (liver and spleen) for measurement of colony-forming units (CFU). For neutralization of IL-22, mice were injected intraperitoneally (i.p.) three times per week with 150μg/mouse per dose monoclonal anti-IL-22 (clone 8E11, Genentech) or mouse IgG1 isotype control (BioXCell/2BScientific). Where indicated, mice were injected i.p. three times per week with 125μg/ml per dose IL-22–Fc (PRO312045, Genentech) or mouse IgG2a isotype control (BioXCell/2BScientific).