Mouse monoclonal antibody against α sma

Rabbit monoclonal antibodies against Oct4 and K6, rabbit polyclonal antibody against TLR4 and mouse monoclonal antibody against α-SMA were purchased from Abcam Biotechnology (Dallas, TX, USA). Rabbit monoclonal antibodies against vimentin, AKT3, PTEN and K17, and mouse monoclonal antibodies against K19 and β-actin were obtained from Cell Signaling Technology (Beverly, MA, USA). Donkey anti-rabbit-IgG and anti-mouse-IgG antibodies (horseradish peroxidase-labeled) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Vimentin antibody used for flow cytometry was purchased from eBioscience Systems (San Diego, CA, USA). Inhibitors of AKT3, TLR4 and PTEN were purchased from MedChem Express (Monmouth, NJ, USA). Trizol reagent was purchased from Invitrogen (Carlsbad, CA, USA). siRNAs of LINC00672 were bought from Sangon Biotech Co., Ltd (Shanghai, China).

For immunofluorescence and immunochemistry, mouse monoclonal antibody against α‐SMA, IgG and IgA, C3, TFRC, IgM or FN (Abcam) and goat polyclonal anti‐mouse secondary antibody and rabbit HRP (Abcam) were used. For the quantitative morphometry, cells stained in 10 randomly selected micrographs were counted using Image ProPlus software (Image‐Pro Plus, Media Cybernetics). For PAS staining, kidney paraffin sections (5 μm) were stained using PAS (ScyTek Laboratories Inc Logan) kits according to the manufacturer's protocols.