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Elisa max deluxe set mouse ige

Manufactured by BioLegend

The ELISA MAX™ Deluxe Set Mouse IgE is a kit designed to measure the concentration of mouse immunoglobulin E (IgE) in biological samples using the enzyme-linked immunosorbent assay (ELISA) technique.

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2 protocols using elisa max deluxe set mouse ige

1

Quantification of Immune Mediators

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The protein levels of IL-4 and IL-5 in BALF were measured by using ELISA MAX™ Deluxe Set Mouse IL-4 and IL-5 (BioLegend, USA) according to the manufacturer’s protocol. For serum IgE analysis, the obtained serum was stored at − 80 °C and measured using ELISA MAX™ Deluxe Set Mouse IgE (BioLegend) according to the manufacturer’s instructions.
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2

Immunoglobulin Profiling in Mice

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Blood was collected and centrifuged at 1,500 × g for 20 min. The obtained serum was stored at −80°C for the immunoglobulin analysis. The level of total IgE in diluted serum was measured using the ELISA MAX Deluxe Set Mouse IgE (BioLegend) kit. The level of OVA-specific IgE was analyzed using the LEGEND MAX Mouse OVA Specific IgE ELISA Kit (BioLegend). Every assay was performed according to the manufacturer’s instructions.
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