Cell lysis buffer

Manufactured by Yeasen

Sourced in China

Cell lysis buffer is a solution designed to disrupt and break down the cell membranes of various cell types, including bacterial, yeast, and mammalian cells. It facilitates the extraction and isolation of cellular components, such as proteins, nucleic acids, and organelles, for downstream analysis and applications.

Automatically generated - may contain errors

Lab products found in correlation

Phosphatase inhibitor cocktail, by Yeasen (1 mentions) Protease inhibitor, by Yeasen (1 mentions) P erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Pparγ, by Cell Signaling Technology (1 mentions) P pka thr197, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Luciferase reporter gene assay kit, by Yeasen (1 mentions)

2 protocols using cell lysis buffer

Adipose Tissue Protein Analysis

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The adipose tissue from T2D mice or 3T3-L1 adipocytes were lysed in cell lysis buffer (Yeasen, China) with phosphatase inhibitor cocktail, protease inhibitor and PMSF. Western blot analysis were performed with 30–50 μg protein using commercially available or donated antibodies to the following: pPKA (Thr197), PKA, pRaf1 (Ser259), Raf1, p ERK1/2 (Thr202/Tyr204), ERK1/2, PPARγ, GLUT4 and GAPDH (Cell Signaling), pPPARγ(Ser273) (Bioss, China), HCAR2 (ABclonal, China), and adiponectin (donated by Dr. Li Zhen of Tsinghua University). Secondary antibodies were obtained from Cell Signaling.

Zhang Y., Li Z., Liu X., Chen X., Zhang S., Chen Y., Chen J., Chen J., Wu F, & Chen G.Q. (2023). 3-Hydroxybutyrate ameliorates insulin resistance by inhibiting PPARγ Ser273 phosphorylation in type 2 diabetic mice. Signal Transduction and Targeted Therapy, 8, 190.

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Dual-Luciferase Assay for Transcriptional Regulation

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The dual-luciferase reporter assay was conducted as reported previously (He et al., 2022) . The effector vectors with or without MYB59 and reporter vectors containing the promoters of SAG18, ICS1, PAL2, and LOX2 were transformed into A. tumefaciens strain GV3101 with pSoup-P19 (Weidi Biotechnology, Shanghai, China). The effector and reporter plasmids were mixed at a ratio of 1:1 (v/v) and injected into the young leaves of 4-week-old N. benthamiana. The infiltrated plants were cultured in a climate chamber under a light (16 h)/dark (8 h) photoperiod at 22°C for three days. The proteins in the transfected leaves were extracted using Cell Lysis Buffer (YEASEN, Shanghai, China) . A luciferase reporter gene assay kit (YEASEN, Shanghai, China) was used to measure firefly luciferase (LUC) and Renilla luciferase (REN, an internal control) using a multifunctional enzyme label instrument (Tecan, Switzerland). Eight independent biological samples were analyzed.

He S., Zhi F., Min Y., Ma R., Ge A., Wang S., Wang J., Liu Z., Guo Y, & Chen M. (2023). The MYB59 transcription factor negatively regulates salicylic acid- and jasmonic acid-mediated leaf senescence. Plant physiology, 192(1).

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