HeLa cells (1 × 104 cells/well) were seeded onto a chambered coverglass (Thermo Fisher, Massachusetts, US) and treated with SAF-Hex at the IC50 concentration for 48 h. Cells were washed with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde (Fujifilm, Tokyo, Japan) for 15 min at room temperature, and permeabilized with 0.05% Triton X-100 (Fujifilm, Tokyo, Japan). Subsequently, cells were incubated with 4′,6-diamidino-2-phenylindole (DAPI) for 15 min and washed with PBS. Finally, fluorescence images were examined using the FLUOVIEW FV10i confocal system (Olympus, Tokyo, Japan).
Fluoview fv10i confocal system
Manufactured by Olympus
Sourced in Japan
The FLUOVIEW FV10i confocal system is a high-performance laser scanning confocal microscope designed for high-resolution imaging of biological samples. It features a compact design, easy-to-use interface, and advanced imaging capabilities.
Automatically generated - may contain errors
3 protocols using fluoview fv10i confocal system
1
Fluorescence Imaging of HeLa Cells
2
Assessing Compound Effects on HeLa Cells
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HeLa cells (approximately 1 × 104 cells/well) were seeded in a 24-well plate and incubated for 1 d. The cells were treated with the compound at the IC50 concentration for 24 h and then incubated with DAPI for 15 min. Fluorescence images were obtained using a FLUOVIEW FV10i confocal system (Olympus, Tokyo, Japan).
3
Mitochondrial Membrane Potential Measurement
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Changes of (Δψ) m were assessed by loading cells with 100nmol/L Tetramethylrhodamine, methyl ester (TMRM) for 30 minutes at 37°C to measure fluorescence in the perigranular mitochondrial region.
Depolarisation of the mitochondria results in redistribution of TMRM from the mitochondria to the cytosol, causing a decrease in mitochondrial fluorescence [44] . Excitation 488nm, emission was detected at >550nm with Olympus Fluoview FV10i confocal system [7] .
Depolarisation of the mitochondria results in redistribution of TMRM from the mitochondria to the cytosol, causing a decrease in mitochondrial fluorescence [44] . Excitation 488nm, emission was detected at >550nm with Olympus Fluoview FV10i confocal system [7] .
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