Size exclusion chromatography was performed on a BioRad NGC Quest 10 Plus Chromatography system using a Superose 6 10/300 GL size exclusion column from Amersham Pharmacia Biotech (Piscataway, NJ). WT and mutant SaPC samples, both at a concentration of 1 mg/mL, were loaded onto the chromatography column with a volume of 1 mL. The protein was eluted using a buffer containing 10 mM Tris-HCl (pH 7.4), 150 mM NaCl, and 2 mM TCEP at a flow rate of 0.3 mL/min. The eluted samples were monitored at 280 nm. The molecular weights and multimerization states of tetrameric WT and dimeric ΔBCΔBCCP PC were previously determined (23 (link)).
Superose 6 10 300 gl size exclusion column
Manufactured by Cytiva
The Superose 6 10/300 GL is a size exclusion chromatography column from Cytiva. It is designed for the separation and purification of biomolecules based on their size and molecular weight. The column features a bed volume of 24 ml and is suitable for use with a wide range of sample types, including proteins, peptides, and other macromolecules.
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2 protocols using superose 6 10 300 gl size exclusion column
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Size Exclusion Chromatography of SaPC Proteins
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Size Exclusion Chromatography of SaPC
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Size exclusion chromatography was performed on a BioRad NGC Quest 10 Plus Chromatography system using a Superose 6 10/300 GL size exclusion column from Amersham Pharmacia Biotech (Piscataway, NJ). WT and mutant SaPC samples, both at a concentration of 1 mg/mL, were loaded onto the chromatography column with a volume of 1 mL. The protein was eluted using a buffer containing 10 mM Tris-HCl (pH 7.4), 150 mM NaCl, and 2 mM TCEP at a flow rate of 0.3 mL/min. The eluted samples were monitored at 280 nm. The molecular weights and multimerization states of tetrameric WT and dimeric ΔBCΔBCCP PC were previously determined (23) (link).
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