Afatinib, adriamycin and cisplatinum were purchased from Meilun Biology Technology Co., Ltd (Dalian, China). Paclitaxel was obtained from Tianfeng Technology Co., Ltd (Xi'an, China). Rhodamine 123, MTT, verapamil, lipopolysaccharide (LPS), PDTC, LY294002 and other chemicals were purchased from Sigma Chemical Co. (St. Louis, MQ, USA).
The monoclonal antibody against ABCB1 was obtained from Santa Cruz Biotechnology Inc. (CA, USA). Antibodies against iκBα, p-iκBα, p65 and Lamin B1 were purchased from Epitomics, Inc. (California, USA). Antibodies against EGFR, p-EGFR, HER-2, p-HER-2, AKT, p-AKT, p38, p-p38, ERK1/2, p-ERK1/2, JNK, p-JNK and GAPDH were obtained from Bioworld Technology, Inc. (Minnesota, USA). The HRP-conjugated goat anti-rabbit secondary antibody and the CY3-conjugated goat anti-rabbit secondary antibody were purchased from Abcam Inc. (Cambridge, MA, USA).
The monoclonal antibody against ABCB1 was obtained from Santa Cruz Biotechnology Inc. (CA, USA). Antibodies against iκBα, p-iκBα, p65 and Lamin B1 were purchased from Epitomics, Inc. (California, USA). Antibodies against EGFR, p-EGFR, HER-2, p-HER-2, AKT, p-AKT, p38, p-p38, ERK1/2, p-ERK1/2, JNK, p-JNK and GAPDH were obtained from Bioworld Technology, Inc. (Minnesota, USA). The HRP-conjugated goat anti-rabbit secondary antibody and the CY3-conjugated goat anti-rabbit secondary antibody were purchased from Abcam Inc. (Cambridge, MA, USA).