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Naocl

Manufactured by Thermo Fisher Scientific
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NaOCl is a chemical compound that is commonly used in various laboratory applications. It is a bleaching agent and disinfectant. NaOCl is the chemical formula for sodium hypochlorite.

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Lab products found in correlation

Cumene hydroperoxide, by Merck Group (1 mentions) Methylhydroquinone, by Merck Group (1 mentions) Mevalonate, by Merck Group (1 mentions) Rpmi medium, by Thermo Fisher Scientific (1 mentions) 4 methylumbelliferyl β d glucuronide hydrate, by Merck Group (1 mentions) N acetylcysteine nac, by Carl Roth (1 mentions) Thiourea, by Carl Roth (1 mentions) Methylglyoxal, by Merck Group (1 mentions) Iodine, by Merck Group (1 mentions) Nh4cl, by Merck Group (1 mentions) Sodium bicarbonate nahco3, by Merck Group (1 mentions) Tempo, by Merck Group (1 mentions)

5 protocols using naocl

1

Comparative Evaluation of Endodontic Irrigants

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Commercial preparations of Biopure MTAD (Dentsply Tulsa Dental, Johnson City, TN) and 2% chlorhexidine gluconate (Dentaclor, Ammdent, Chandigarh, India) were obtained.
The 2.5% NaOCl was freshly prepared from 4% NaOCl (Fischer Scientific, Mumbai, India). iodine potassium iodide was freshly prepared by dissolving 2% iodine (Merck, Mumbai, India) in a 4% aqueous solution of potassium iodide (Rankem, Delhi, India).
Biopure MTAD was used with 1.3% NaOCl as per the manufacturer's instructions. We prepared fresh solutions of 1.3% NaOCl from 4% NaOCl (Fischer Scientific, Mumbai, India).
Juneja N, & Hegde M.N. (2014). Comparison of the Antifungal Efficacy of 1.3% NaOCl/MTAD with Other Routine Irrigants: An Ex-Vivo Study. International Scholarly Research Notices, 2014, 575748.
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2

Chlorination of Water Samples

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A stock solution of sodium hypochlorite (NaOCl, Fisher Scientific, Pittsburgh, PA, USA) was used for chlorination. It was standardized according to Standard Method 4500B [24 ]. The water samples were dosed with a chlorine concentration that allowed the free residual chlorine concentration to be in the range of 0.2 to 1.0 mg·L−1 after 48 h of incubation at room temperature according to the procedures delineated in Standard Method 2350B and Standard Method 5710C [24 ]. Chlorine residuals were analyzed using the DPD ferrous titrimetric method in Standard Method 4500F [24 ].
Wang Y.H, & Chen K.C. (2014). Removal of Disinfection By-Products from Contaminated Water Using a Synthetic Goethite Catalyst via Catalytic Ozonation and a Biofiltration System·. International Journal of Environmental Research and Public Health, 11(9), 9325-9344.
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3

Bacterial Strain Cultivation and Assays

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A summary of the bacterial strains and oligos used in this study is listed in Supplementary Tables 1, 2, respectively.
B-medium is a modified LB medium suitable for staphylococci cultivation by adding 1 g/L potassium phosphate (Brückner, 2006 (link)). In cultivation steps, the ratio of the bacterial suspension to the total volume of the flask was less than or equal to 1:3 to ensure sufficient aeration.
RPMI medium (catalog number 72400021) was purchased from LIFE Technologies. Formaldehyde (FA), diamide, and NaOCl was bought from Fisher Scientific, MP Biomedicals, and Alfa Aesar, respectively. 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG), methylhydroquinone (MHQ), methylglyoxal (MG), H2O2, cumene hydroperoxide (CHP), and mevalonate were obtained from Sigma-Aldrich. Thiourea, N-acetylcysteine (NAC), and catalase was purchased from Carl Roth, Hölzel Diagnostika, and MP Biomedicals, respectively. Stressors were dissolved in sterilized Milli-Q water for β-galactosidase and survival assays.
Ibrahim E.S, & Ohlsen K. (2022). The Old Yellow Enzyme OfrA Fosters Staphylococcus aureus Survival via Affecting Thiol-Dependent Redox Homeostasis. Frontiers in Microbiology, 13, 888140.
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4

Monochloramine Disinfection Experiments

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For monochloramine experiments, PBS of pH 8.0 was used. Monochloramine solutions were prepared by mixing the appropriate volume of 1.91% NH4Cl (Sigma-Aldrich, UK) and 14.5% NaOCl (Alfa Aesar, UK) solutions. A series of monochloramine concentrations from 0.001 to 10 mg L−1 were prepared in PBS. The remaining protocol was the same as that used for chlorine (described above).
Khan S., Beattie T.K, & Knapp C.W. (2017). The use of minimum selectable concentrations (MSCs) for determining the selection of antimicrobial resistant bacteria. Ecotoxicology (London, England), 26(2), 283-292.
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5

Synthesis of F-18 Fluoroglucosamine from FDG

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We synthesized FGA from commercially available 2-deoxy-2-[18F]fluoro-D-glucose (FDG). The pre-formulation data leading up to a kit has been published elsewhere [11 (link)]. A typical kit vial contained a lyophilized mixture of 0.8 mg of 2,2,6,6-tetramethyl-1-piperidinyloxy or TEMPO (Sigma-Aldrich, St. Louis, MO), 8 mg of sodium bromide (NaBr, Mallinckrodt, Paris, Kentucky, USA), and 24 mg sodium bicarbonate (NaHCO3, Sigma-Aldrich). For conversion of FDG into FGA, FDG was introduced into the vial in presence of 20 μL NaOCl (14% available chlorine, Alfa Aesar, Tewksbury, MA). After 5 min, the reaction was stopped and the mixture was neutralized by 1.5 mL of 0.2 M hydrochloric acid (HCl). The resultant product was drawn into a syringe for injection via a 0.2 μm syringe filter. The presence of FGA and FDG in the final preparation was monitored by thin-layered chromatography (TLC) as described elsewhere [11 (link)].
Houson H., Mdzinarishvili A., Gali H., Sidorov E, & Awasthi V. (2020). PET detection of cerebral necrosis using an infarct-avid agent 2-deoxy-2-[18F]fluoro-D-glucaric acid (FGA) in a mouse model of the brain stroke. Molecular imaging and biology, 22(5), 1353-1361.
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