Spleen and lymph node cells (5-10 × 10 6 /ml) of diabetic and TSA-treated mice and NOD.scid mice transferred with Th1 or Th17 cells were cultured with PMA (100 ng), ionomycin (one μg), and brefeldin A (Sigma, 5 μg) for 4 hr, as described earlier (Jayaraman et al., 2017) . Cells were stained with CD3e eFluor450, fixed, permeabilized, blocked with 10% goat serum, and stained with anti-IFN-γ-FITC (clone XMG1.2), anti-IL-17A-PE (clone eBio17B7, eBioscience), or APC-conjugated monoclonal anti-IL-10 antibody (clone JES5-16E3, eBioscience). Cells were analyzed on a BD flow cytometer using FlowJo 6.3.4 software (Treestar), and typical cytograms were obtained, as shown previously (Jayaraman et al., 2017) .
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted July 25, 2021. ; https://doi.org/10.1101/2021.07.24.453657 doi: bioRxiv preprint
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted July 25, 2021. ; https://doi.org/10.1101/2021.07.24.453657 doi: bioRxiv preprint