Phospholipid extrusion membranes

Unless otherwise stated all chemicals were purchased from Sigma-Aldrich (St. Louis, MO or Stockholm, Sweden). DMEM, FBS, penicillin-streptomycin, Lipofectamine 2000, Optimem, Trypsin-EDTA, ProLong Gold Antifade Reagent with DAPI, Proteinase K (25530–049), and the Virapower Adenoviral Expression System were obtained from Invitrogen (Carlsbad, CA). Plus slides (J3800AMNZ), Coomassie Brilliant Blue, and the Immobilon-FL PVDF membrane were purchased from Thermo Fisher Scientific (Rockford, IL). The QuickTiter Adenovirus Titer Elisa kit was purchased from Cell Biolabs (San Diego, CA). Antisedan, Temgesic, and the Fentanyl/Dormitor mixture were obtained from Apoteksbolaget, (Stockholm, Sweden). The Vectastain ABC kit was purchased from Vector Laboratories, Inc. (Burlingame, CA), and 3,3′-diaminobenzidine (DAB Safe) was obtained from Saveen Werner (Limhamnsvägen, Sweden). Isopropyl β-D-1-thiogalactopyranoside and ampicillin were purchased from Gold Biotechnology (St. Louis, MO). L-α-phosphatidylcholine (egg PC), L-α-phosphatidylglycerol (egg PG), and phospholipid extrusion membranes were purchased from Avanti Polar Lipids (Alabaster, AL). Iodixanol and the enhanced chemifluorescence (ECF) substrate were obtained from GE Life Sciences (Pittsburgh, PA). 100 kDa spin filters and 10 kDa concentration filters were obtained from Millipore (Billerica, MA or Solna, Sweden).

Unless otherwise stated, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Isopropyl β-D-1-thiogalactopyranoside (IPTG), ampicillin, and phenylmethylsulfonyl fluoride (PMSF) were purchased from Gold Biotechnology (St. Louis, MO). L-α-phosphatidylcholine (egg PC), L-α-phosphatidylglycerol (egg PG), and phospholipid extrusion membranes were purchased from Avanti Polar Lipids (Alabaster, AL). Dulbecco’s minimal essential media (DMEM), penicillin-streptomycin, the ViraPower Adenoviral Expression System, and LR clonase II were obtained from Invitrogen (Carlsbad, CA). Fetal bovine serum (FBS) was obtained from Corning Life Sciences (Corning, NY). Iodixanol and ECF substrate were obtained from GE Life Sciences (Pittsburgh, PA). 100 kDa spin filters were purchased from Millipore (Billerica, MA). Bicinchoninic acid (BCA) assay reagents and Coomassie Brilliant Blue were purchased from Thermo Scientific (Rockford, IL).