We trained the mice to run back and forth on a 96-cm-long, 5-cm-wide linear track elevated 33 cm above the floor18 (link). We placed the track in a square enclosure (160 × 160 cm) within the recording room. The walls of this 190-cm-high enclosure were curtains with visual cues of different colors and patterns (distal cues). Distinct visual cues were also scattered along the walls (4 cm high) of the linear track (proximal cues). To record mouse behavior, we used an overhead camera (DFK 33G445, The Imaging Source, Germany), which was synchronized with the integrated microscope. Both pre-training and imaging sessions consisted of five to seven 3-min-long trials, with an inter-trial interval of 3 min Ca2+ imaging was performed at 20 or 10 Hz in CA1 or ACC, respectively. The number of pre-training days and the behavior of the mice on the track were similar for the presented analyses (six pre-training sessions for recordings from the ACC and eight for recordings from the CA1; the number of track traversals was 68 ± 7 for CA1 and 64 ± 9 for ACC).
Dfk 33g445
Manufactured by Imaging Source
Sourced in Germany
The DFK 33G445 is a USB 3.0 industrial color camera from The Imaging Source. It features a 1/3" CMOS sensor with a resolution of 2048 x 1536 pixels and a frame rate of up to 60 frames per second.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dfk 33g445
1
Spatial Cognition in Mice: Linear Track Protocol
2
Two-Photon Imaging of GCaMP6 in Freely Behaving Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To perform time-lapse imaging in freely behaving mice using an integrated miniature fluorescence microscope (nVistaHD, Inscopix), we followed a previously established protocol [25] . Mice were water-restricted and maintained above 80% of their initial weight. We habituated the mice to human handling by allowing them to walk on the experimenters' hands. At least three weeks after guide-tube implantation, mice were imaged under isoflurane anesthesia using a two-photon microscope (Ultima IV, Bruker, Germany). We inserted a microendoscope consisting of a single gradient refractive index lens (1 mm diameter, Inscopix) into the guide tube and examined Ca 2+ indicator expression and tissue health. We selected for further imaging only those mice that exhibited homogeneous GCaMP6 expression and appeared to have healthy tissue. For the selected mice, we then affixed the microendoscope within the guide tube using an ultraviolet-curing adhesive (Norland, NOA81, Edmund Optics, Barrington, NJ). Next, we attached the microscope's base plate to the dental acrylic cap using a light-cured adhesive (Flow-It ALC, Pentron, Orange, CA). To record mouse behavior, we used an overhead camera (DFK 33G445, The Imaging Source, Germany), which we synchronized with the integrated microscope. Ca 2+ imaging was performed at 20 Hz.
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