Sodium di hydrogen phosphate

AChE, acetylthiocholine iodide (ATCI), 5,5-dithio-bis-2-nitrobenzoic acid (DTNB), reduced glutathione (GSH), pyridostigmine bromide, 1-chloro-2,4-dinitrobenzene (CDNB) and Folin’s reagent were procured from Sigma chemical Co. (St. Louis, MO, USA). Trichloroacetic acid (TCA), hydrogen peroxide, sodium di-hydrogen phosphate, sodium hydroxide and sodium carbonate were procured from Sisco Research Laboratory, Mumbai, India. 2,3-dimethylmaleic anhydride is isolated and characterised (purity, 96%) from root stock of Colocasia esculenta var esculenta (L.) Schott as reported earlier^{21 (link)}.

Chlorogenic acid, MPTP, and normal goat serum (NGS) were bought from Sigma–Aldrich (St. Louis, MO, United States). Acetic acid, ammonium chloride, bovine serum albumin (BSA), potassium chloride, disodium hydrogen phosphate, reduced nicotinamide adenine dinucleotide phosphate (NADPH), and sodium dihydrogen phosphate were procured from Sisco Research Laboratories (SRL, Mumbai, India). Protein estimation kit by Bradford GeNeiTM, hydrogen peroxide (H₂O₂), and potassium dichromate were purchased from Merck (Darmstadt, Germany), and sodium dodecyl sulphate (SDS), thiobarbituric acid (TBA), DABCO, and Griess reagent were obtained from HiMedia (Mumbai, India). Paraformaldehyde and sodium nitrite were bought from Lobachemie, India. Primary antibodies for TH (SC-25269), iNOS (SC-651), and glial fibrillary acidic protein (GFAP; SC-33673) were purchased from Santa Cruz, Biotechnology (Santa Cruz, CA, United States), and the primary antibodies for TNF-α (ab1793) and NF-κB (ab16502) were obtained from Abcam Life Science, Biogenuix Medsystems Pvt. Ltd. (New Delhi, India), and secondary fluorescent-tagged antibodies such as Cy2 conjugated and Cy3 conjugated for immunohistochemistry were bought from Merck Millipore and Chemicon, respectively.