Log In Sign up
The largest database of trusted experimental protocols

Giemsa staining solution

Manufactured by Muto Pure Chemicals
Visit Supplier
Sourced in Japan

Giemsa staining solution is a laboratory reagent used for the differential staining of blood smears and other biological samples. It contains a mixture of methylene blue, eosin, and azure dyes that selectively stain various cellular components, enabling the visualization and identification of different cell types under a microscope.

Automatically generated - may contain errors

Lab products found in correlation

12 well culture plate, by Thermo Fisher Scientific (1 mentions) Complete mesencult expansion medium, by STEMCELL (1 mentions) Bz x810 microscope, by Keyence (1 mentions)

Spelling variants of this product

Giemsa solution (8 citations) Giemsa (2 citations)

2 protocols using giemsa staining solution

1

Mesenchymal Cell Expansion Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated cells were seeded onto a 12-well culture plate (1000 cells/well, Thermo Fisher Scientific) and cultured using the Complete MesenCult expansion medium (Stem Cell Technologies) for 7 days. Cells were stained with Giemsa staining solution (Muto pure chemicals).
Tamura S., Mukaide M., Katsuragi Y., Fujii W., Odaira K., Suzuki N., Tsukiji N., Okamoto S., Suzuki A., Kanematsu T., Katsumi A., Takagi A., Ikeda K., Ueyama J., Hirayama M., Suzuki-Inoue K., Matsushita T., Kojima T, & Hayakawa F. (2022). Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development. The Journal of Biological Chemistry, 298(5), 101833.
+ Open protocol
+ Expand
2

Giemsa Staining of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were washed with PBS and fixed with methanol for 5 min at 24 °C. The cells were dried for 10 min at 24 °C and immersed in 20-fold diluted Giemsa Staining Solution (MUTO PURE CHEMICALS Co., Ltd., Tokyo, Japan) for 20 min at 24 °C. The cells were washed with tap water and rinsed with double-distilled water. After drying, images of the cells were captured at a magnification of 20× using a BZ-X810 microscope (KEYENCE CORPORATION, Osaka, Japan). Representative images are shown.
Matsumoto T., Matsumoto J., Matsushita Y., Arimura M., Aono K., Aoki M., Terada K., Mori M., Haramaki Y., Imatoh T., Yamauchi A, & Migita K. (2023). Bortezomib Increased Vascular Permeability by Decreasing Cell–Cell Junction Molecules in Human Pulmonary Microvascular Endothelial Cells. International Journal of Molecular Sciences, 24(13), 10842.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!