Cepharanthine was purchased from Shiji-Aoke (Beijing, China), cell counting kit-8 was bought from APExBio (Houston, TX, United States), DMEM, penicillin, streptomycin, fetal bovine serum (FBS) and PBS were obtained from Gibco Life Technologies (Grand Island, NY, United States). Anti-GAPDH, P62 and anti-rabbit IgG HRP-linked antibody were obtained from Cell Signaling Technology, Inc., (Beverly, MA, United States). Cathepsin B antibody was purchased from the Santa Cruz Biotechnology (Santa Cruz, CA, United States). Primary antibodies against LC3 were obtained from Abcam (Cambridge, MA, United States), anti-rabbit HRP-conjugated secondary antibody and anti-mouse HRP-conjugated secondary antibody were purchased from Cell Signaling Technology (Boston, MA, United States).
Primary antibodies against lc3
Manufactured by Abcam
Sourced in United States
Primary antibodies against LC3 are used to detect the microtubule-associated protein 1A/1B-light chain 3 (LC3), which is a widely used marker for autophagosomes. These antibodies can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the role of autophagy in different biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions)
Anti mouse hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Cathepsin b antibody, by Santa Cruz Biotechnology (1 mentions)
Cell counting kit 8 (cck8), by Apexbio (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Caspase 3, by Abcam (1 mentions)
2 protocols using primary antibodies against lc3
1
Cepharanthine-Mediated Autophagy Regulation
2
Investigating LPS-Induced Autophagy and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LPS was obtained from Sigma-Aldrich; primary antibodies against LC-3 and Caspase-3 were obtained from Abcam Corporation.
Ninety-six healthy male Sprague-Dawley (SD) rats (180–200 g) were obtained from the Laboratory Animal Research Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China). Animals were maintained under specific pathogen-free conditions (temperature: 22 ± 2°C, relative humidity: 40%–80%), and allowed free access to standard laboratory food and water.
Ninety-six healthy male Sprague-Dawley (SD) rats (180–200 g) were obtained from the Laboratory Animal Research Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China). Animals were maintained under specific pathogen-free conditions (temperature: 22 ± 2°C, relative humidity: 40%–80%), and allowed free access to standard laboratory food and water.
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