Beyoecl plus p0018

DMEM and McCoy's 5A medium were purchased from GE Healthcare Life Sciences, HyClone Laboratories. Histostain-Plus Kits (SP-9001, SP-9002) and Mouse Anti-β actin mAb(TA-09) were purchased from ZSGB-Bio, Beijing, China. Luteolin (≥98%, L9283) and ellagic acid (≥95%, E2250) were purchased from Sigma-Aldrich (USA). DAB Horseradish Peroxidase Color Development Kit (P0203), BeyoECL Plus (P0018) and Hematoxylin Staining Solution (C0107) were purchased from Beyotime Institute of Biotechnology. MMP2 (BMO569) and MMP9 (PB0709) were purchased as primary antibodies from Boster Biological Technology Co., LTD.⁴⁶ (link) HRP-linked rabbit- and mouse-anti IgG (7074s, 7076s) were chosen from CST (USA). Mouse MMPs ELISA Kit (DM-X6142, DM-X6008) was purchased from Baomanbio, Shanghai, China.

CM was purchased from Xinjiang Nanshan Pasture (Urumqi, China). The milk was collected from Camelus bactrianus. After traditional pasteurization, it was transported to the laboratory via food cold chain logistics within 48 h. Cytokines TNF-α, IL-1β, IL-6, and IL-10 kits were obtained from Wuhan ColorfulGene Biological Technology Company. SOD, MDA, and GSH kits were acquired from Nanjing Jiancheng Bioengineering Institute. Antibodies for Western blot, namely, HMGB(166525-1-Ig) and TLR4 (19811-1-AP) were purchased from ProteinTech Group (Chicago, IL, USA). MyD88 (YT2928) was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA). IKKβ (D30C6) was obtained from Cell Signaling Technology (Shanghai, China). NF-κB (ARG65677) was acquired from Bio-Platform Technology Company (Shanghai, China). β-actin (BM 0627) was bought from Boster Biological Technology (Wuhan, China). BeyoECL Plus (P0018) and BCA Protein Assay Kit were purchased from Beyotime Company (Shanghai, China).