The human cervix epithelial adenocarcinoma cell line HeLa was purchased from the American Type Culture Collection, and the liver carcinoma cell line HepG2 from the European Collection of Authenticated Cell Cultures (Sigma-Aldrich Chemie N.V., Zwijndrecht, the Netherlands). General cell culture supplies were from Gibco (Life Technologies Europe B.V., Bleiswijk, the Netherlands) if not indicated otherwise: HeLa cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) + 4.5 g/l d -glucose + l -glutamine + 25 mm HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) without pyruvate (42430-025) to which MEM nonessential amino acids (11140-035) were added and 10% fetal bovine serum (Sigma, F7524, Sigma-Aldrich Chemie N.V). HepG2 cells were cultured in Eagle's Minimum Essential Medium (EMEM) + Earle’s salts without l -glutamine (21090-022) to which MEM nonessential amino acids (11140-035) were added and 10% fetal bovine serum (American Type Culture Collection, ATCC 30-2020, LGC Standards GmbH, Wesel, Germany).
For transfections, HeLa and HepG2 cells were seeded at approximately 80% density in 96-well plates (Greiner Bio-One 655180). HeLa cells were transfected using the TransIT-HeLaMONSTER Transfection Kit (MIR 2904) and HepG2 cells with TransIT-LT1 Transfection Reagent (MIR 2304) applying the standard protocols (both from Mirus Bio LLC, Sopachem B.V., Ochten, the Netherlands).
For transfections, HeLa and HepG2 cells were seeded at approximately 80% density in 96-well plates (Greiner Bio-One 655180). HeLa cells were transfected using the TransIT-HeLaMONSTER Transfection Kit (MIR 2904) and HepG2 cells with TransIT-LT1 Transfection Reagent (MIR 2304) applying the standard protocols (both from Mirus Bio LLC, Sopachem B.V., Ochten, the Netherlands).