Serum ATX concentrations were determined with a specific two-site enzyme immunoassay using the automated immunoassay analyzer AIA-2000 system (Tosoh Co., Tokyo, Japan), as described previously[36 (link)]. To prepare the 2-site immunoassay, R10.23 was digested with pepsin and the purified F(ab)2 form using phenyl-5PW (Tosoh Co.) hydrophobic column chromatography in order to avoid the nonspecific binding of human antibodies against various animal IgG in human specimens, like human anti-mouse antibodies. Magnetic beads were coated with R10.23 F(ab)2 and placed in the reaction cup, and 35 ng of alkaline phosphatase-labeled R10.21 in assay buffer (5% BSA, 5% sucrose, 10 mmol/L Tris-HCl, 10 mmol/L MgCl2, pH 7.4) was added to the reaction cup. ATX assay reagent was prepared by immediate freeze-dry procedure of the reaction cup. The ATX assay reagent thus prepared can be used with AIA-system.
Aia 2000 system
Manufactured by Tosoh
Sourced in Japan
The AIA-2000 system is an automated immunoassay analyzer designed for clinical diagnostic laboratories. It is capable of performing a wide range of immunoassay tests, including those for hormones, tumor markers, and infectious diseases. The system features automated sample handling, incubation, and detection capabilities to provide consistent and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using aia 2000 system
1
Automated Enzyme Immunoassay for ATX
2
Cryogenic Serum ATX Antigen Measurement
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Blood samples were obtained and immediately stored at −20 °C until testing. Serum ATX antigen concentrations were simultaneously measured using cryogenically stored serum samples by a specific two-site enzyme immunoassay with an AIA-2000 system (Tosoh Co., Tokyo, Japan) as described previously47 (link).
3
Fibrosis Biomarkers in Liver Disease
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The fibrosis-4 (FIB-4) index and AST-to-platelet ratio index (APRI) were respectively calculated as: age (years) × AST (IU/L) / (platelet count [10 9 /L] × ALT [IU/L] 1/2 ) 17 and (AST / upper limit of normal; 40 IU/L) × (100 / platelet count [10 9 /L]) 18, 19 . Isolated blood samples were immediately stored at -20°C until testing. Serum autotaxin (ATX) antigen concentration was simultaneously measured using frozen serum samples by a specific two-site enzyme immunoassay with an AIA-2000 system (Tosoh Co., Tokyo, Japan) as described previously [20] [21] [22] . The recently established macrophage galactose-specific lectin-2 binding protein glycosylation isomer (M2BPGi) fibrosis marker was quantified as earlier described 23 .
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