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2 protocols using tu212

1

Cultivation of Highly and Less Malignant Human LSCC Cell Lines

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Human LSCC cell lines, TU212 (highly malignant) and TU686 (less malignant), were obtained from Shanghai Cell Bank, Chinese Academy of Sciences. Both TU212 and TU686 cell lines were routinely cultured in Dulbecco’s modified Eagle medium (DMEM, #11965118; Gibco, New York, USA), supplemented with 10% fetal bovine serum (FBS, #16000; Gibco), 100 µ/ml penicillin, and 100 mg/ml streptomycin (#15140-122; Gibco) in a humidified atmosphere containing 5% CO2 at 37°C.
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2

Culturing Human Laryngeal and Bronchial Cell Lines

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Three human laryngeal cancer cell lines (SNU899, TU212, and Hep-2) and one normal human bronchial epithelial cell line BEAS-2B were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, P.R. China). Cells were cultured in RPMI-1640 medium (Gibco, Los Angeles, CA, USA) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin. All the cells were maintained at 37°C in a humidified incubator containing 5% CO2.
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