LPS (Escherichia coli 055:B5) were obtained from Aladdin (Beijing, China). De Man, Rogosa, and Sharpe (MRS) was from Solarbio Ltd. (Solarbio, Beijing, China). Total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), ALT, AST, MDA, T-AOC, SOD, GSH-Px, CAT, TNF-α, transforming growth factor (TGF)-β, IL-1β, IL-6, IL-17, IL-10, IL-22, and LPS kits were obtained from MY BioSource Co., Ltd. (San Diego, CA, USA).
Gsh px
Manufactured by MyBioSource
Sourced in United States
GSH-Px is a laboratory equipment used for the quantitative determination of glutathione peroxidase (GPx) activity. GPx is an enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides, playing a crucial role in the antioxidant defense system.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 6 (il 6), by MyBioSource (2 mentions)
Lipopolysaccharides (lps), by MyBioSource (1 mentions)
Il 1β, by MyBioSource (1 mentions)
Il 10, by MyBioSource (1 mentions)
Tnf α, by MyBioSource (1 mentions)
Il 17, by MyBioSource (1 mentions)
Il 22, by MyBioSource (1 mentions)
Interleukin 4 (il 4), by MyBioSource (1 mentions)
Tbars, by Cayman Chemical (1 mentions)
2 protocols using gsh px
1
Evaluation of LPS-Induced Metabolic Effects
2
Antioxidant and Inflammatory Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The serum and milk α-tocopherol and selenium (as selenium-binding protein 1) concentrations were assessed using commercially available ELISA kits (Mybiosource, USA) according to manufacturer's instructions (Jovanović et al. 2013 ). The microplates were read at 533 nm and 450 nm wavelengths, respectively using spectrophotometer (Thermo Fisher, USA). The intra and inter CV of the kit were <10% and the recovery rate was 95-98%.
The serum and milk concentrations of TBARS (Cayman Chemical, USA), NO, SOD, GSH-Px, IL-4 and IL-6 (Mybiosource, USA) were determined using commercially available ELISA kits according to manu-facturer's instructions (Ibrahim et al. 2016 ). The microplates were read at 412-550 nm wavelength using spectrophotometer (Thermo Fisher, USA). The intra and inter CV of the kits were ˂10%. The recovery rate was 92%-98%.
The serum and milk concentrations of TBARS (Cayman Chemical, USA), NO, SOD, GSH-Px, IL-4 and IL-6 (Mybiosource, USA) were determined using commercially available ELISA kits according to manu-facturer's instructions (Ibrahim et al. 2016 ). The microplates were read at 412-550 nm wavelength using spectrophotometer (Thermo Fisher, USA). The intra and inter CV of the kits were ˂10%. The recovery rate was 92%-98%.
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