Innotest phoshotau 181p

CSF levels of T-tau, P-tau and Aβ42 were quantified with commercially available ELISAs; Innotest® hTau Ag, Innotest® phoshoTau (181P) [26 (link), 27 (link)] and Innotest® β-amyloid 1–42 [28 (link)] (Fujirebio Europe, Gent, Belgium). Aβ1–38 (Aβ38), Aβ1–40 (Aβ40) and Aβ42 were analysed using a MSD Multi-Spot Assay System (Meso Scale Discovery, Rockville, MA, USA) with 6E10 (BioLegend, San Diego, CA, USA) as the detection antibody. The Meso Scale Discovery analyses, which were applied to a subset of patients in the Norwegian cohort, were carried out according to the manufacturers’ procedures.

CSF levels of t-tau, p-tau, and Aβ42 were quantified with commercially available ELISAs; Innotest® hTau Ag, Innotest® phoshoTau (181P), and Innotest® β-amyloid 1–42 as previously described [28 (link)–30 (link)] (Fujirebio Europe, Gent, Belgium). CSF Aβ peptide levels were determined with CSF Aβ_1–38 (Aβ38), Aβ_1–40 (Aβ40), and Aβ_1–42 (Aβ42) MSD Triplex assay (Meso Scale Discovery, Rockwilly, MA, USA). All these analyses were performed at the Clinical Neurochemistry Laboratory at Sahlgrenska University Hopsital, Mölndal, Sweden. CSF cut-off for pathological level was < 530 pg/ml (Aβ42+/−), ≥ 60 pg/ml (p-tau+/−), and > 350 pg/ml (t-tau +/−) [31 (link)].