The following conjugated Abs were used for cell surface staining: CD3-Pacific Blue (clone UCHT1), CD4-Alexa Fluor 700 (clone RPA-T4), CD8-APC-H7 (clone SK1), CD160-Alexa Fluor 647 (clone BY55), T-cell immunoglobulin and mucin-domain containing-3 (TIM-3)-PE-CF594 (clone 7D3), 2B4-FITC (clone 2–69) (BD Biosciences, San Jose, CA), and programmed cell death protein-1 (PD-1)-PE (clone J105) (Thermo Fisher Scientific). Conjugated Abs for intracellular staining included the following: cytotoxic T lymphocyte antigen-4 (CTLA-4)-PE-Cy5 (clone BNI3), granzyme B-PE-CF594 (clone GB11), interferon-gamma (IFN-γ)-PE-Cy7 (clone B27), interleukin-2 (IL-2)-APC (clone MQ1-17H12), tumor necrosis factor-alpha (TNF-α)-Alexa Fluor 488 (clone MAb11) (BD Biosciences, San Diego, CA), and perforin-PE (clone B-D48) (Abcam, Cambridge, UK). All conjugated Abs were titrated and multicolor panels for flow cytometry assays were approached as previously reported [38 (link)]. The following purified (No azide/Low endotoxin) Abs were used in cultured cells: CD28 (clone CD28.2) and CD49d (clone 9F10) (BD Biosciences).
Perforin pe clone b d48
Manufactured by Abcam
Perforin-PE (clone B-D48) is a laboratory reagent used for the detection and quantification of perforin, a cytolytic protein found in cytotoxic T cells and natural killer cells. This product is a fluorescently labeled monoclonal antibody that binds to the perforin protein, allowing for its identification and measurement in various experimental and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 alexafluor700, by BD (2 mentions)
Pd 1 pe clone j105, by Thermo Fisher Scientific (2 mentions)
Granzyme b pe cf594, by BD (2 mentions)
Tnf α alexa fluor 488 clone mab11, by BD (2 mentions)
Cd28 clone cd28.2, by BD (2 mentions)
Cd49d clone 9f10, by BD (2 mentions)
Cd8 apc h7, by BD (2 mentions)
Cd3 pacific blue, by BD (2 mentions)
Ifn γ pe cy7 clone b27, by BD (1 mentions)
Ifn γ pe cy7, by BD (1 mentions)
2 protocols using perforin pe clone b d48
1
Multicolor Flow Cytometry Staining
2
Comprehensive Immune Cell Phenotyping
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For staining of the cell surface, the following conjugated antibodies were used: CD3-Pacific Blue (clone UCHT1), CD4-Alexa Fluor 700 (clone RPA-T4), CD8-APC-H7 (clone SK1), CD160-Alexa Fluor 647 (clone BY55), TIM-3-PE-CF594 (clone 7D3), 2B4-FITC (clone 2–69) (BD Biosciences, San Jose, CA), and PD-1-PE (clone J105) (Thermo Fisher Scientific). Intracellular staining was performed with the following antibodies: CTLA-4-PE-Cy5 (clone BNI3), granzyme B-PE-CF594 (clone GB11), IFN-γ-PE-Cy7 (clone B27), IL-2-APC (clone MQ1-17H12), TNF-α-Alexa Fluor 488 (clone MAb11) (BD Biosciences, San Diego, CA), and perforin-PE (clone B-D48) (Abcam, Cambridge, UK). All conjugated antibodies were titrated as previously reported [29 (link)]. The multicolor panels used for the flow cytometry assays were utilized according to the fluorescence minus one control (FMO) method as recommended [29 (link)]. In addition, the following purified NA/LE antibodies were used for cell culture: CD28 (clone CD28.2) and CD49d (clone 9F10) (BD Biosciences).
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