Cell pellets were extracted twice in chloroform/methanol (2∶1, v:v) in the presence of tricosanoic acid methyl ester (Sigma Aldrich, Milan Italy), as internal standard. Fatty acids of cell total lipid extract were analysed by GC-MS on a capillary column (FFAP, 60 m×0.32 µm×0.25 mm, Hewlett Packard, Palo Alto, CA, USA), as previously reported [36] (link). Results of experiments performed in each donor (n = 3) in triplicate are given as mean percentage ± S.D.
Tricosanoic acid methyl ester
Manufactured by Merck Group
Sourced in Ireland
Tricosanoic acid methyl ester is a long-chain fatty acid ester. It is a colorless, waxy solid used as a reference standard and chemical intermediate in analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Trichloroacetic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions)
Methanol, by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Sucrose, by Merck Group (2 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions)
Potassium hydroxide, by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
Mannitol, by Merck Group (2 mentions)
Heptane, by Merck Group (2 mentions)
Acetyl chloride, by Merck Group (2 mentions)
Disodium sulphate, by Merck Group (2 mentions)
Supelco 37 fame standard, by Merck Group (2 mentions)
2 thiobarbituric acid, by Merck Group (2 mentions)
1 1 3 3 tetraethoxypropane, by Merck Group (2 mentions)
Millipore water purification system, by Merck Group (2 mentions)
N pentane, by Thermo Fisher Scientific (2 mentions)
3 protocols using tricosanoic acid methyl ester
1
Fatty Acid Profiling in Cells
2
Lipid Extraction and Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Trichloroacetic acid, HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)), EGTA (EGTAzic acid), disodium sulphate, hydrochloric acid, potassium hydroxide, 1,1,3,3-tetraethoxypropane , 2-thiobarbituric acid , methanol, heptane, Supelco-37 FAME standard, tricosanoic acid methyl ester, as well as acetyl chloride, sucrose, mannitol and potassium chloride were purchased from Sigma-Aldrich (Arklow, Co.
Wicklow, Ireland). N-pentane as well as sodium chloride was purchased from Fisher Scientific (Dublin, Ireland). Ultra-pure water (18.2 MΩ cm -1 ) was generated in-house using a Millipore water purification system (Millipore, Cork, Ireland) . All chemical were GC grade.
Wicklow, Ireland). N-pentane as well as sodium chloride was purchased from Fisher Scientific (Dublin, Ireland). Ultra-pure water (18.2 MΩ cm -1 ) was generated in-house using a Millipore water purification system (Millipore, Cork, Ireland) . All chemical were GC grade.
3
Lipid Extraction and Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Trichloroacetic acid, HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)), EGTA (EGTAzic acid), disodium sulphate, hydrochloric acid, potassium hydroxide, 1,1,3,3-tetraethoxypropane , 2-thiobarbituric acid , methanol, heptane, Supelco-37 FAME standard, tricosanoic acid methyl ester, as well as acetyl chloride, sucrose, mannitol and potassium chloride were purchased from Sigma-Aldrich (Arklow, Co.
Wicklow, Ireland). N-pentane as well as sodium chloride was purchased from Fisher Scientific (Dublin, Ireland). Ultra-pure water (18.2 MΩ cm -1 ) was generated in-house using a Millipore water purification system (Millipore, Cork, Ireland) . All chemical were GC grade.
Wicklow, Ireland). N-pentane as well as sodium chloride was purchased from Fisher Scientific (Dublin, Ireland). Ultra-pure water (18.2 MΩ cm -1 ) was generated in-house using a Millipore water purification system (Millipore, Cork, Ireland) . All chemical were GC grade.
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