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2 protocols using cefixime

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Antibiotic susceptibility testing of urine isolates

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Positive urine cultures were processed for antimicrobial susceptibility testing on Mueller-Hinton agar plates, using the Kirby-Bauer disk diffusion method, according to the CLSI guideline (14 ). Gram-negative bacteria were evaluated against amikacin, nitrofurantoin, cotrimoxazol, cephalotin, gentamycin, ceftriaxon, nalidixic, cefixime, ciprofloxacin, tetracyclin, pipracillin and imipenem (Mast Co., England).
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2

Antibiotic Susceptibility Profiling of Isolates

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Antibiotic susceptibility of all isolates to ampicillin, amoxicillin-clavulanate, cefoxitin, cefixime, ceftazidime, ceftriaxone, cefotaxime, cefepime, ciprofloxacin, ofloxacin, nalidixic acid, aztreonam, tetracycline, gentamicin, trimethoprim/sulfamethoxazole, nitrofurantoin, and imipenem (Mast Co., UK) was carried out on Muller-Hinton agar (Oxoid Co., UK) using the disk diffusion method as recommended by the Clinical and Laboratory Standards Institute (CLSI).10
E. coli ATCC 25,922 was used as the quality control strain for antibacterial susceptibility testing. The isolates non-susceptible to ≥1 agent at least three of antibiotic categories were defined as MDR.11 (link)
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