Nf533 17

Manufactured by Thorlabs

Sourced in United States, United Kingdom

The NF533-17 is a single-channel, wide-bandwidth amplifier with a gain range of x1 to x100. It is designed to amplify and condition low-level signals. The device features adjustable gain, offset, and bandwidth settings to optimize the signal processing for the specific application.

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Lab products found in correlation

8 protocols using nf533 17

Optical Exciton Tuning of MoS2 Nanowires

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An inverted microscope (Nikon TiE) equipped with a 100× oil objective (Nikon, NA 0.5–1.3) was used. An oil-immersion dark-field condenser with a NA of 1.20–1.43 was used to focus the white illuminating light onto the MoS₂ nanowire from the top. A linear polarizer (Thorlabs) was used to filter unpolarized white light into linearly polarized light. The forward scattering signal from the MoS₂ nanowire was directed to an optical spectrometer (Andor) with a 500 nm grating. Background spectra were also recorded and subtracted to obtain the scattering signal of the MoS₂ nanowires. The scattering spectra were finally normalized by the incident light. In the optical exciton tuning experiments, a 532 nm laser (Coherent, Genesis MX STM-1 W) was directed to the microscope to heat the MoS₂ nanowire. A notch filter (NF 533–17, Thorlabs) was used to block the laser signal from entering the spectrometer.

Li J., Yao K., Huang Y., Fang J., Kollipara P.S., Fan D.E, & Zheng Y. (2022). Tunable Strong Coupling in Transition Metal Dichalcogenide Nanowires. Advanced materials (Deerfield Beach, Fla.), 34(34), e2200656.

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Single-particle optical characterization of WS2 and a-SiNS:H

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An inverted microscope (Nikon TiE) and a spectrograph (Andor Shamrock 303i-B) with an EMCCD (Andor Newton DU970P) are used for the experiments. A glass coverslip is always applied on the sample to work with an oil immersion objective (Nikon Plan Fluor 100X Oil, NA 0.5). As for the measurement in water, a drop of deionized water is encapsulated between the substrate and coverslip, immersing the WS₂ and a-SiNS:Hs. In the single particle scattering measurement (Fig. 2A), an oil immersion dark-field condenser (Nikon NA 1.20-1.43) is used to focus the white illuminating light (halogen light source, 12 V, 100 W) onto the sample from the top. Forward scattering signal is collected by the 100X oil immersion objective. In the photoluminescence (PL) measurement, a 532 nm laser (Coherent, Genesis MX STM-1 W) is expanded with a 5X beam expander (Thorlabs, GBE05-A) and directed to the microscope. The 100X oil immersion objective is used to focus the laser on the sample and to collect the PL signal. A notch filter (Thorlabs, NF533-17) is used to block the 532 nm laser signal towards the spectrometer. The signal collection window is limited to the single-particle area. The pure WS₂ PL signal is measured five times at different positions surrounding the a-SiNS:H and averaged.

Fang J., Yao K., Zhang T., Wang M., Jiang T., Huang S., Korgel B.A., Terrones M., Alù A, & Zheng Y. (2022). Room-temperature observation of near-intrinsic exciton linewidth in monolayer WS2. Advanced materials (Deerfield Beach, Fla.), 34(15), e2108721.

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Single-cell Laser-based Manipulation

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The setup used for single cell manipulation experiments has been described before^{15 (link)}. A weakly focused pulsed Nd:YAG laser beam (λ = 532 nm, pulse width = 850 ps, repetition rate = 20.25 kHz; Horus, France) was guided onto the sample from above. The spot diameter was approx. 60 µm. An inverse epifluorescence microscope (Axio Observer, HBO 50 fluorescence excitation lamp, HAL100 brightfield lamp) was used to monitor fluorescence signals and obtain bright field images. Images were acquired with a cooled CCD-camera (ProgRes MF cool, Jenoptik, Germany). An OD6 notch filter (NF533-17, Thorlabs) protected the camera from laser irradiation. We used a 480 nm ± 15 nm bandpass filter for Fluo 4 excitation; emission was evaluated with a 520 nm longpass filter.

Johannsmeier S., Heeger P., Terakawa M., Kalies S., Heisterkamp A., Ripken T, & Heinemann D. (2018). Gold nanoparticle-mediated laser stimulation induces a complex stress response in neuronal cells. Scientific Reports, 8, 6533.

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Optical Fiber-based NV Magnetometer

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The optical fiber-based system is presented in Fig. 7. A green laser (gem 532, 250 mW, Laser Quantum, UK) was injected into the bulk diamond through an isolator (IO-3-532-LP, Thorlabs, USA) a beam splitter (VA5–532/M, Thorlabs, USA), and a 2 × 1 fiber coupler (TM105R5F1A, Thorlabs, USA: 105 μm core diameter, 50:50 split). The red luminescence from the diamond was measured using a photodiode (DET100A2, Thorlabs, USA) through a bifurcated optical fiber bundle, the longpass filter (FELH0600, Thorlabs, USA: >600 nm) and notch filter (NF533-17, Thorlabs, USA: 533 nm) in a filter box (FOFMF/M, Thorlabs, USA). The detected luminescence was measured using a lock-in amplifier (SR830, Stanford Research Systems, USA) through a transimpedance amplifier (TIA60, Thorlabs, USA). A MW generator (MG3740A, Anritsu, Japan) and amplifier (ZHL-16W-43-S+, +45 dB, Mini-circuits, USA) were applied to the NV via a copper thin plate (the width and length were approximately 5 mm, and the thickness was 0.04 mm). The bulk diamond and the edge of the optical fiber were connected using an optical adhesive (Norland Optical Adhesive 68, Norland Products Inc., USA) that was cured under ultraviolet light exposure. The core diameter of the fiber was 105 μm (NA = 0.22), and the estimated active area of the diamond was approximately 100 μm × 100 μm × 500 μm.Figure 7

Detailed optical fiber-based system.

Kuwahata A., Kitaizumi T., Saichi K., Sato T., Igarashi R., Ohshima T., Masuyama Y., Iwasaki T., Hatano M., Jelezko F., Kusakabe M., Yatsui T, & Sekino M. (2020). Magnetometer with nitrogen-vacancy center in a bulk diamond for detecting magnetic nanoparticles in biomedical applications. Scientific Reports, 10, 2483.

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Lifetimes of Excited CdTe Quantum Dots by TCSPC

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The lifetimes of excited CdTe QDs were recorded by using a time-correlated single-photon counting (TCSPC) technique by using a home-built, all-reflective epifluorescence setup^{52 (link)}. The sample solution was excited at the front of a 1 cm quartz cuvette using a 532-nm pulsed diode laser (PicoQuant, LDH-P-FA-530B). The emission was then collected and filtered with a 550-nm long-pass dichroic beamsplitter (Thorlabs, DMLP550R), a 550-nm long-pass filter (Thorlabs, FELH0550) and a 532-nm notch (Thorlabs, NF533–17), and finally focused onto a Si single-photon avalanche photodiode diode (Micro Photon Devices, PD-050-CTD). The TCSPC traces were constructed using HydraHarp 400 and the corresponding software (Picoquant). All measurements were carried out at room temperature. The experimental data were fitted with bi-exponential decays as follows:

I (t) = \sum_{i = 1}^{2} α_{i} exp (- \frac{t}{τ_{i}})

where τ_i are the decay times, α_i represent the amplitudes of the components at t = 0, The average lifetimes (

\bar{τ}

) were then calculated as follows⁵³:

\bar{τ} = \frac{α_{1} τ_{1}^{2} + α_{2} τ_{2}^{2}}{α_{1} τ_{1} + α_{2} τ_{2}}

Guan X., Erşan S., Hu X., Atallah T.L., Xie Y., Lu S., Cao B., Sun J., Wu K., Huang Y., Duan X., Caram J.R., Yu Y., Park J.O, & Liu C. (2022). Maximizing light-driven CO2 and N2 fixation efficiency in quantum dot-bacteria hybrids. Nature catalysis, 5(11), 1019-1029.

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Laser-Based Stimulation and Imaging of HEK293 Cells

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For HEK293 stimulation, the quasi-cw OPO output at 1,440 nm was loosely focused with a 1-m-focal-length lens into a spot 670 μm in diameter (Fig. 2b), providing a uniform irradiation of a large number of TRPA-expressing cells close to the beam centre. Glass-bottom Petri dishes with cultured cells were placed on a stage of an upright multiphoton microscope (Thorlabs) equipped with XLUMPLFLN objective (NA1.05, Olympus). Continuous-wave laser sources with wavelengths of 473 and 532 nm and an average power up to 50 mW were used for R-GECO1, EGFP, GCaMP6s and tdTomato visualization. Fluorescence from EGFP and GCaMP6s was filtered with an FELH0500 (Thorlabs) low-frequency filter and an FF01-510/42 (Semrock) bandpass filter. The signal from R-GECO1.1 and tdTomato was filtered with a NF533-17 (Thorlabs) notch filter and an FF01-607/70 (Semrock) bandpass filter. Fluorescence images were recorded using a cooled CCD camera 4070M-GE-TE (Thorlabs) with 4 × 4 binning and a 900-ms exposure.

Ermakova Y.G., Lanin A.A., Fedotov I.V., Roshchin M., Kelmanson I.V., Kulik D., Bogdanova Y.A., Shokhina A.G., Bilan D.S., Staroverov D.B., Balaban P.M., Fedotov A.B., Sidorov-Biryukov D.A., Nikitin E.S., Zheltikov A.M, & Belousov V.V. (2017). Thermogenetic neurostimulation with single-cell resolution. Nature Communications, 8, 15362.

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Fluorescence Spectroscopy using Diamond Sensor

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The setup is sketched in Fig. 3. A 532 nm laser beam was focused into the sensing diamond using a plano-convex lens with a focal length of 𝑓 = 8 mm. Behind the diamond we placed initially another lens and a notch filter to separate out green light from the (infra)red fluorescence light emitted from the diamond detected with a charge-coupled device (CCD) camera. That way we were able to verify that the diamond was well centred illuminated with the 532 nm light. The camera was positioned on the back side producing the characteristic cross shape shown in Fig. 1 (c). This shape originates from reflections of the side surfaces of the sensing diamond and allows for precise positioning of the diamond relative to the laser beam using a XYZ-stage. After alignment, the optics at the back side were replaced with the same type of aspheric condenser lens with 𝑓 =8 mm focal length, a notch filter and a photodiode. The fluorescence was compared in both front and back direction simultaneously. Integrated over the expected fluorescence spectrum the notch filter (Thorlabs NF 533-17) transmits about 2% more than the dichroic mirror (Thorlabs DMLP-567 ), negligible within the measurement error.

Omar M., Conta A., Westerhoff A., Hasse R., Chatzidrosos G., Budker D, & Wickenbrock A. (2023). Diamond-optic enhanced photon collection efficiency for sensing with nitrogen-vacancy centers. Optics letters, 48(10).

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Laser Ablation of Plant Roots

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Laser ablation was performed using a different system [28] . A 532 nm Q-switched pulsed laser source (Continuum minilite II) and an aspherical lens (C280TMD-C, f = 18.40 mm, NA = 0.15) were used to focus the beam on the sample. The laser provided high peak power of 5.0 × 10 6 W to 8.3 × 10 6 W in pulses of nanoseconds in duration, delivering precise dissection with minimal thermal damage to the surrounding root tissues. The aspherical lens of the ablation instrument was chosen to achieve a small focal spot at the required working distance in a lens, whilst also remaining tolerant of the high peak powers of the laser pulses. Targeting of the ablation was achieved by moving root samples anchored on the translation stage. The (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted December 19, 2021. ; https://doi.org/10.1101/2021.12.18.473189 doi: bioRxiv preprint 4 imaging system consisted of a Köhler illumination, an imaging objective (TU Plan ELWD 20X, Nikon, UK), a notch filter (NF533-17, Thorlabs, UK), a tube lens (LA1509-A, Thorlabs, UK) and a CCD camera (Guppy F-046, Allied Vision, Germany) acquiring images at 10 frames per second.

Ge S., Wright K.M., Humphris S., Dupuy L., & MacDonald M.P. (2021). In situcontrol of root–bacteria interactions using optical trapping in transparent soil.

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