KA (Tocris), D-AP5 (Cayman Chemicals), MPEP (Cayman Chemicals), JNJ-16259685 (Tocris, JNJ), GYKI-53655 (hellobio), NASPM (Cayman Chemicals, NASPM), GF(Tocris), H-89 (Cayman Chemicals), UBP-302 (Tocris, UBP), DCZ-dihydrochloride (Cayman Chemicals), nifedipine (Tocris), and ML-218 (Tocris) were dissolved in either deionized water or DMSO (JNJ, GF, H-89, UBP, nifedipine, and ML-218) and stored in aliquots at −20°C. Aliquots were dissolved in ACSF immediately before the experiments. Experiments involving nifedipine were conducted in the dark.
Ml 218
Manufactured by Bio-Techne
Sourced in United Kingdom
ML-218 is a benchtop flow cytometer designed for automated cell analysis. It features advanced optics and fluidics systems to provide high-sensitivity detection and accurate cell population identification. The instrument is capable of detecting multiple fluorescent parameters simultaneously, making it suitable for a variety of applications in cell biology and immunology research.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Nifedipine, by Merck Group (1 mentions)
5 dodecanoylaminofluorescein di β d galactopyranoside c12fdg, by Thermo Fisher Scientific (1 mentions)
ω conotoxin mviic, by Bio-Techne (1 mentions)
Fura 2 acetoxymethyl ester fura 2 am, by Merck Group (1 mentions)
Clarity max western ecl substrate, by Bio-Rad (1 mentions)
Sh sy5y cells, by European Collection of Cell Cultures (1 mentions)
Thapsigargin tg, by Abcam (1 mentions)
Nifedipine, by Bio-Techne (1 mentions)
Naspm, by Bio-Techne (1 mentions)
D ap5, by Cayman Chemical (1 mentions)
Jnj16259685, by Bio-Techne (1 mentions)
Ubp302, by Bio-Techne (1 mentions)
2 protocols using ml 218
1
Pharmacological Tools in Neuroscience Research
2
SH-SY5Y Cell Culture and Characterization
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SH-SY5Y cells were purchased from European Collection of Cell Cultures (ECACC) and distributed by Sigma-Aldrich (St. Louis, MO, USA); Phoenix-AMPHO HEK293 were from American Type Culture Collection (ATCC); all-trans-retinoic acid, human brain-derived neurotrophic factor (BDNF), collagen (type I), Dulbecco’s modified Eagle’s medium (DMEM), DMEM:F-12 Ham medium, and nifedipine were purchased from Sigma-Aldrich (St. Louis, MO, USA); fura-2-acetoxymethyl ester (fura-2-AM) and BTP2 were from Merck Millipore (Darmstadt, Germany); thapsigargin (Tg) was from Abcam Biochemicals (Cambridge, UK); ML 218 and ω-conotoxin MVIIC were from Tocris Bioscience (Bristol, UK); rhodamine 123, tetramethylrhodamine methyl ester (TMRM), Hoechst 33258, Hoechst 33342, and 5-dodecanoylaminofluorescein di-β-d -galactopyranoside (C12FDG) were from Thermo Fisher Scientific (Waltham, MA, USA); Clarity Max™ Western ECL substrate was from Bio-Rad (Hercules, CA, USA).
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