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Anti granzyme k pe

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Anti-granzyme K-PE is a laboratory reagent used for the detection and analysis of granzyme K, a serine protease involved in cell-mediated cytotoxicity. This product is a conjugate of an anti-granzyme K antibody with the fluorescent dye Phycoerythrin (PE), allowing for the visualization and quantification of granzyme K-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Anti granzymeb pacific blue, by BioLegend (2 mentions) Anti cd62l pe cy5 clone dreg 56, by BioLegend (2 mentions) Anti cd3 af700 clonehit3a, by BioLegend (2 mentions) Anti cd45ra bv605, by BioLegend (2 mentions) Anti granzyme a percp cy5, by BioLegend (2 mentions) Bv650 streptavidin, by R&D Systems (2 mentions) Anti granzyme m efluor660, by Thermo Fisher Scientific (2 mentions) Bv510 streptavidin, by BioLegend (2 mentions) Anti cd8 fitc clone sk1, by BioLegend (2 mentions)

2 protocols using anti granzyme k pe

1

Assessing CD8+ T Cell Cytolytic Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rested ex vivo CD8+ T cells were assessed for CMV and
HIV specificity using HLA-matched tetramers. Tetramers were made via
biotinylated monomer conjugation to BV510 streptavidin (BioLegend). Monomers
were obtained from Dr. Soren Buus (University of Copenhagen). The A02-NV9
tetramer was used to stain for CMV-specificity while pools of A02-SL9, B27-KK10,
B53-QW9, B53-YY9, B57-IW9(p24), B57-IW9(RT), B57-KF11, B57-QW9, and B57-TW10
tetramers were used to stain for HIV-specificity. Subsequent surface stains
included anti-CD62L-PE-Cy5 (clone DREG-56; BioLegend), anti-CD3-AF700 (clone
HIT3a; BioLegend), anti-CD8-FITC (clone SK1; BioLegend), anti-CD45RA-BV605
(clone HI100; BioLegend), and LIVE/DEAD Blue. Following permeabilization, the
cells were intracellular stained using anti-perforin PE/Cy7 (clone B-D48;
BioLegend), anti-granzyme A-PerCp/Cy5.5 (clone CB9; BioLegend), anti-granzyme
B-Pacific Blue (clone GB11; BioLegend), anti-granzyme H (biotinylated and
secondary stained with BV650 streptavidin) (polyclonal antibody, R&D),
anti-granzyme K-PE (clone GM26E7; BioLegend), and anti-granzyme M-eFluor660
(clone 4B2G4; eBioscience). The cells were analyzed by flow cytometry.
Naïve CD8+ T cells within the mixed population of
CD8+ T cells were used as internal gating controls for
perforin and each granzyme as these cells do not express these effector
molecules.
Clayton K.L., Collins D.R., Lengieza J., Ghebremichael M., Dotiwala F., Lieberman J, & Walker B.D. (2018). Resistance of HIV-infected macrophages to CD8 T lymphocyte-mediated killing drives immune activation. Nature immunology, 19(5), 475-486.
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2

Assessing CD8+ T Cell Cytolytic Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rested ex vivo CD8+ T cells were assessed for CMV and
HIV specificity using HLA-matched tetramers. Tetramers were made via
biotinylated monomer conjugation to BV510 streptavidin (BioLegend). Monomers
were obtained from Dr. Soren Buus (University of Copenhagen). The A02-NV9
tetramer was used to stain for CMV-specificity while pools of A02-SL9, B27-KK10,
B53-QW9, B53-YY9, B57-IW9(p24), B57-IW9(RT), B57-KF11, B57-QW9, and B57-TW10
tetramers were used to stain for HIV-specificity. Subsequent surface stains
included anti-CD62L-PE-Cy5 (clone DREG-56; BioLegend), anti-CD3-AF700 (clone
HIT3a; BioLegend), anti-CD8-FITC (clone SK1; BioLegend), anti-CD45RA-BV605
(clone HI100; BioLegend), and LIVE/DEAD Blue. Following permeabilization, the
cells were intracellular stained using anti-perforin PE/Cy7 (clone B-D48;
BioLegend), anti-granzyme A-PerCp/Cy5.5 (clone CB9; BioLegend), anti-granzyme
B-Pacific Blue (clone GB11; BioLegend), anti-granzyme H (biotinylated and
secondary stained with BV650 streptavidin) (polyclonal antibody, R&D),
anti-granzyme K-PE (clone GM26E7; BioLegend), and anti-granzyme M-eFluor660
(clone 4B2G4; eBioscience). The cells were analyzed by flow cytometry.
Naïve CD8+ T cells within the mixed population of
CD8+ T cells were used as internal gating controls for
perforin and each granzyme as these cells do not express these effector
molecules.
Clayton K.L., Collins D.R., Lengieza J., Ghebremichael M., Dotiwala F., Lieberman J, & Walker B.D. (2018). Resistance of HIV-infected macrophages to CD8 T lymphocyte-mediated killing drives immune activation. Nature immunology, 19(5), 475-486.
+ Open protocol
+ Expand

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