Sugarcane molasses medium: Sugarcane molasses (Zhongfuxin, Guangxi, China) 20 BX°, (NH4)2SO4 (Macklin, Shanghai, China) 0.1%, KH2PO4 (Macklin, Shanghai, China) 0.1%, MgSO4 (Macklin, Shanghai, China) 0.04% (w/v).
Nitrogen-rich medium (w/v): Soybean meal (Macklin, Shanghai, China) 5%, peptone 2%, yeast extract 1%.
YEPD medium (w/v): Glucose 2%, peptone 2%, yeast extract 1%.
GPYM medium (w/v): Glucose 4%, peptone 0.5%, yeast extract 0.5%, MgSO4·7 H2O 0.1%, malt extract (Solarbio, Beijing, China) 0.5%.
About 100 mL of Glucose medium, sugarcane molasses medium, and nitrogen-rich medium were prepared, respectively, in 250 mL conical flask, and 10% (v/v) yeast seed liquids were inoculated, respectively, and cultivated at 28°C, 150 rpm for 3 days.