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2 protocols using peptone

1

Microbial Growth Media Preparation

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Glucose medium (w/v): Glucose (Aladdin, Shanghai, China) 10%, peptone (Oxoid, UK) 2%, yeast extract (Oxoid, UK) 1%.
Sugarcane molasses medium: Sugarcane molasses (Zhongfuxin, Guangxi, China) 20 BX°, (NH4)2SO4 (Macklin, Shanghai, China) 0.1%, KH2PO4 (Macklin, Shanghai, China) 0.1%, MgSO4 (Macklin, Shanghai, China) 0.04% (w/v).
Nitrogen-rich medium (w/v): Soybean meal (Macklin, Shanghai, China) 5%, peptone 2%, yeast extract 1%.
YEPD medium (w/v): Glucose 2%, peptone 2%, yeast extract 1%.
GPYM medium (w/v): Glucose 4%, peptone 0.5%, yeast extract 0.5%, MgSO4·7 H2O 0.1%, malt extract (Solarbio, Beijing, China) 0.5%.
About 100 mL of Glucose medium, sugarcane molasses medium, and nitrogen-rich medium were prepared, respectively, in 250 mL conical flask, and 10% (v/v) yeast seed liquids were inoculated, respectively, and cultivated at 28°C, 150 rpm for 3 days.
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2

Anthocyanin and Lysine Protocol

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Anthocyanin was purchased from Tianjin Jianfeng Natural products R & D Co., Ltd. (Tianjin, China); lysine was purchased from Shanghai aladdin Biochemical Technology Co., Ltd. (Shanghai, China); calcium chloride, agar, cholesterol, and peptone were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China); NaOH and NaCl were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); an Oil Red O staining kit was purchased from Beijing Leagene Biotechnology Co., Ltd. (Beijing, China); and TG assay kits, NEFA assay kits, MDA, and CAT assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All chemicals were used without purification.
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