High-performance liquid chromatography grade solvents, including methanol, acetonitrile, and water, were purchased from J.T. Baker (PA, USA). Formic acid, nicotinamide, adenosine monophosphate, LysoPC, and LysoPE were obtained from Sigma-Aldrich (MO, USA) and Avanti Polar Lipids (AL, USA).
Lysope
Manufactured by Merck Group
Sourced in United States
LysoPE is a laboratory reagent used for the analysis of phospholipids. It functions as a lysolipid, facilitating the identification and quantification of phosphatidylethanolamine (PE) lipids in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine monophosphate, by Merck Group (1 mentions)
Nicotinamide, by Merck Group (1 mentions)
Lysopc, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Chloramphenicol, by Merck Group (1 mentions)
Ricinoleic acid, by Merck Group (1 mentions)
Hipersolv chromanorm, by Avantor (1 mentions)
3 protocols using lysope
1
Quantification of Lipid Metabolites
2
Fibroblast Cell Culturing and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fibroblast cells were obtained from patient- and gender-/age-matched control from skin punch biopsies. The skin biopsy from individual II-I was taken at the time of her hospitalization at age 10 while undergoing an operative procedure. Fibroblast cells were cultured in MEM, without glutamine (Gibco) but supplemented with 2 mM of L-glutamine (Gibco) and 10% heat-inactivated FBS (Gibco). Fibroblast cells were detached from 10-cm culture dishes (Fisher) using Trypsin–EDTA (0.25%) and passed in a 1:3 ratio three times per week. For confocal microscopy experiments, fibroblast cells were seeded at 20,000 cells per well using 24-well plates on glass cover slips (12-545-81; Thermo Fisher Scientific) overnight. Fibroblast cells were treated with 50 μM lyso-PE (89576-29-4; Sigma-Aldrich) in ethanol and/or 20 mM 2-deoxyglucose (sc-202010; Santa Cruz) for 48 h.
3
High-Quality Pasteurized Milk Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol, acetonitrile, formic acid, and ammonium formate were all of HPLC grade (HiPerSolv Chromanorm) and purchased from VWR International Ltd. Analytical standards of chloramphenicol, oleic acid, ricinoleic acid, and 18:1 lysophosphatidylethanolamine (Lyso PE) were purchased from Sigma Aldrich, and water was purified using a Milli-Q system (Millipore).
High-quality pasteurized milk was produced according to Italian Ministerial Decree No. 185 (D. M. 185/91, 1991) , which establishes the general requirements for raw milk intended for the production of high-quality pasteurized milk: raw milk must have a fat content of at least 3.5% and a protein content of 32 g/L. Concerning pasteurization, a typical example of effective applied time-temperature operative conditions is 77°C for 15 s. High-quality pasteurized milk samples were collected from batches that arrived daily, after milking and pasteurization, in our laboratory (1 sample per day was collected for each of the working days). Milk samples were supplied by a single trusted dairy, located between Cremona and Parma provinces, Italy. To increase the variability of the model, the sampling period lasted 6 wk, and the sample collection for validation was executed 4 mo later, to increase the variability and the seasonality of the set and the correspondent robustness of the study.
High-quality pasteurized milk was produced according to Italian Ministerial Decree No. 185 (D. M. 185/91, 1991) , which establishes the general requirements for raw milk intended for the production of high-quality pasteurized milk: raw milk must have a fat content of at least 3.5% and a protein content of 32 g/L. Concerning pasteurization, a typical example of effective applied time-temperature operative conditions is 77°C for 15 s. High-quality pasteurized milk samples were collected from batches that arrived daily, after milking and pasteurization, in our laboratory (1 sample per day was collected for each of the working days). Milk samples were supplied by a single trusted dairy, located between Cremona and Parma provinces, Italy. To increase the variability of the model, the sampling period lasted 6 wk, and the sample collection for validation was executed 4 mo later, to increase the variability and the seasonality of the set and the correspondent robustness of the study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!