Diabetes antigen control human low high

Laboratory analyses were performed at Linköping University, Sweden. Blood and serum samples were collected at baseline and after 1, 2, 3, 6, and 15 months. Samples were drawn during the morning hours and peripheral blood mononuclear cells (PBMCs) were isolated within 24 h using Leucosep (Greiner Bio One) according to the manufacturer’s instructions.
Serum C-peptide was determined using a solid phase-two side enzyme immunoassay (Mercodia, Uppsala), and results were validated with the inclusion of a Diabetes Antigen Control Human (Low/High) (Mercodia, Uppsala, Sweden). Inter and intra assay variation were 7% and 4% respectively.

Laboratory analyses were performed at Linköping University, Sweden. Blood and serum samples from all the DIAGNODE-1 participants were collected at baseline and after 15, 30 months. Additionally, samples from the three patients in DIAGNODE Extension were collected at 31.5, 33.5, 36.5 and 42.5 months. Samples were drawn during the morning hours, and peripheral blood mononuclear cells (PBMCs) were isolated within 24 h using Leucosep (Greiner Bio One) according to the manufacturer’s instructions.
Analysis of serum C-peptide was performed using a solid-phase two-sided enzyme immunoassay (Mercodia, Uppsala). Results for each assay were validated with the inclusion of a Diabetes Antigen Control Human (Low/High) (Mercodia, Uppsala, Sweden). The assay is calibrated against the international reference reagent for C-peptide IRR C-peptide 84/510. Inter- and intra-assay variations were 6.6% and 3.5%, respectively.