High-virulence T. gondii RH strains (genotype I; ATCC, USA) were maintained in human foreskin fibroblasts (HFFs; ATCC, USA) in complete Dulbecco’s modified Eagle medium (DMEM; Gibco™, China) supplemented with 10% fetal bovine serum (FBS; Gibco™, USA) and 1% penicillin-streptomycin solution (100×; Thermo Fisher Scientific, USA) at 37 °C in a 5% CO2 incubator.
Penicillin streptomycin solution 100
Manufactured by Thermo Fisher Scientific
Sourced in United States
Penicillin–Streptomycin Solution 100× is a culture medium supplement used in cell and tissue culture applications. It contains a combination of the antibiotics penicillin and streptomycin at a 100× concentrated stock solution.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Hepg2, by American Type Culture Collection (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Fetal calf serum fbs, by Sartorius (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Milli q water, by Merck Group (1 mentions)
Kanamycin, by Thermo Fisher Scientific (1 mentions)
Millipore purification system, by Merck Group (1 mentions)
Isopropyl β d thiogalactoside iptg, by Thermo Fisher Scientific (1 mentions)
Doxorubicin hydrochloride dox, by Meilun (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Mhcc97h, by American Type Culture Collection (1 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Female balb c mice, by Taconic Biosciences (1 mentions)
Resazurin, by Thermo Fisher Scientific (1 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Dcfda cellular ros detection assay kit, by Abcam (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
11 protocols using penicillin streptomycin solution 100
1
Culturing High-virulence Toxoplasma gondii
2
Engineered Nanocarriers for Cancer Therapy
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Three genes encoding HFn, HFn‐PAS, and HFn‐PAS‐RGDK, respectively, were synthesized by the BGI Company (China). HFn‐PAS was constructed by fusing GFLG ASPAAPAPASPAAPAPSAPAASPAAPAPASPAAPAPSAPA GGSGG to HFn subunit C‐terminus through a flexible 15‐residue linker (GGGSGGGTGGGSGGG). Forty underlined residues constitute PAS peptide and GFLG is a Cathepsin B cleavable site. HFn‐PAS‐RGDK has four extra C‐terminus residues, RGDK, compared with HFn‐PAS. Three types of genes were inserted between NdeІ and XhoІ site of pET30a plasmid, and plasmids were then transformed into Escherichia coli (E. coli) BL21 (DE3) using heat‐shock transformation method.
Tryptone, yeast extract, Isopropyl β‐d ‐thiogalactoside (IPTG) and kanamycin were purchased from Thermo Scientific (USA). Chromatography columns were bought from GE healthcare (USA). Doxorubicin hydrochloride (DOX) was purchased from Dalian Meilun Biotechnology (China). MCF7 cell line was kindly offered by Dr Nicholas Eyre from the University of Adelaide. RPMI‐1640 medium, penicillin‐streptomycin solution (100×), fetal bovine serum (FBS), and 0.25% trypsin‐EDTA (1×) solution were purchased from Thermo scientific (USA). All other reagents of analytical grade were purchased from Chem‐supply (Australia). Milli Q water was obtained from a Millipore purification system (Merck, USA) and used in all buffers and solutions.
Tryptone, yeast extract, Isopropyl β‐
3
Culturing Human Cell Lines
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Human HCC cell lines MHCC-97H, HepG2, and HEK293T were obtained from the American Type Culture Collection. These cells were tested for mycoplasma contamination and cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (TIANHANG) and 1% penicillin–streptomycin solution (100 ×) (Thermo Fisher Scientific) in humidified air with 5% CO2 at 37 °C.
4
Dextran-Coated Iron Nanoparticle Cytotoxicity
5
Pharmacological modulation of AMPK and autophagy
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Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin solution (100×) were from Gibco (Life Technologies, CA, USA). Fetal calf serum (FBS) was from Biological Industries (Kibbutz Beit-Haemek, Israel). Florfenicol was from Aladdin (Shanghai, China). DMSO, D-galactose and N-acetyl-L-cysteine (NAC) were from Sigma (St. Louis, MO, USA). AMPK inhibitor (compound C) and Bafilomycin A1 (Baf-A1) were from Selleck Chemicals (Houston, TX, USA).
6
Culturing Human Breast Cancer Cell Lines
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Human BC cell lines, MCF-7, MDA-MB-231 and SK-BR-3, were obtained from the American Type Culture Collection (ATCC) and maintained as a monolayer culture in DMEM medium (Dulbecco’s Modified Eagle’s Medium; HyClone, Waltham, MA, USA) supplemented with 1% Penicillin–Streptomycin Solution 100× (Gibco), 1% Amphotericin B Solution (250 μg/mL) (Sigma-Aldrich, Saint Louis, MO, USA), and 10% Fetal Bovine Serum (FBS) (Lonza, Basel, Switzerland). Cells were cultured at 37 °C in a 5% CO2 atmosphere. Cell lines were maintained in these conditions until all experiments were carried out.
7
Culture and Expansion of Cell Subpopulations
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Cell subpopulations were cultured in suspension on ultra-low attachment plates in spheres medium DMEM-F12 (Dulbecco’s Modified Eagl’s Medium/Nutrient Mixture F-12 Ham; Sigma-Aldrich), supplemented with 1% Penicillin–Streptomycin Solution 100× (Gibco), 1 mg/mL hydrocortisone (Sigma-Aldrich), 4 ng/mL heparin (Sigma-Aldrich), 1× ITS (Gibco), 1× B27 (Gibco), 10 ng/mL EGF (Sigma-Aldrich) and 10 ng/mL FGF (Sigma-Aldrich).
8
Establishing Cell Lines for Transporter Studies
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An MTT kit was purchased from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM) in high glucose, penicillin-streptomycin solution (100×), 0.25% Trypsin-EDTA, and PBS were acquired from Gibco Company (Grand Island, NY). DMSO was bought from Guangzhou Chemical Reagent Factory (Guanzhou, China). OCT2, Pgp, Mrp1, and Mrp2 mouse monoclonal antibodies and anti-mouse IgG HRP-conjugated antibody were purchased from Abcam (Cambridge, UK). GAPDH rabbit monoclonal antibody and anti-rabbit IgG HRP-conjugated antibody were obtained from Cell Signaling Technology (Beverly, MA). OCT2-pCMV6-AC-GFP, Mrp2-pCMV6-AC-GFP, ABCB1-pCMV6-AC-GFP, and pCMV6-AC-GFP plasmids and Mega Tran 1.0 transfection reagent were purchased from OriGene Technologies Company (USA). Aminoglycoside antibiotic (G418) was purchased from Guangzhou Xueyou Biotech Limited Company (Guanzhou, China).
9
Stem Cell Culture Reagents
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Minimum essential medium α (αMEM) and penicillin–streptomycin solution (100×) were from Gibco (Life Technologies, CA). Bovine calf serum (CS) and fetal calf serum (FBS) were from Biological Industries (Kibbutz BeitHaemek, Israel). FLO was from Aladdin (Shanghai, China). DMSO was from Sigma (St. Louis, MO, United States). Sox2 (66411-1-Ig), Oct4 (11263-1-AP), β-catenin (66379-1-Ig), and β-actin (66009-1-Ig) were from Proteintech (Wuhan, China).
10
Photocrosslinkable Hydrogel for Cell Culture
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NIPAM was supplied by Sigma-Aldrich (St. Louis, MO, USA) and further purified by recrystallization from a mixed solution of toluene/n-hexane (6 : 1, v/v). OMAPOSS was purchased from Hybrid Plastics (Hattiesburg, MS, USA) and used as received. 1,4-Dioxane and ethanol were purchased from Titan Scientific (Shanghai, China). Irgacure 2959 was purchased from Wako Pure Chemical Industries (Osaka, Japan). Alpha-minimum essential medium (α-MEM) and phosphate buffer saline (PBS) were purchased from HyClone (South Logan, UT, USA). Fetal bovine serum (FBS), penicillin/streptomycin solution (100×) and trypsin was purchased from Gibco (Grand Island, NY, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Ethylenediamine tetraacetic acid (EDTA), trypan blue, calcein-AM, propidium iodide (PI), N,N′-methylenebisacrylamide (MBA), OEGMA (Mn = 500 g mol−1), rhodamine-phalloidin and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Distilled water was prepared using a Milli-Q system (EMD Millipore, Billerica, MA, USA) and used in all experiments.
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