Att20 cell line

All the samples were obtained following transsphenoidal surgery performed at Beijing Tiantan Hospital from June 2018 to June 2019. Fresh tumor samples were stored in liquid nitrogen. 45 corticotroph adenomas, 79 gonadotroph adenomas, 17 lactotroph adenomas and 31 somatotroph adenomas from the study population (age range, 20–75 years) were diagnosed according to the 2017 World Health Organization classification of tumors of endocrine organs. The study protocols were approved by the Internal Review Board of Beijing Tiantan Hospital, which was affiliated with Capital Medical University, and conformed to the ethical guidelines of the Declaration of Helsinki (No. KY2016-035-01).
The GH3 and MMQ cell lines (ATCC, Manassas, VA, USA) cultured in a humidified incubator at 37° C and 5% CO₂ in F-12K medium (ATCC) supplemented with 2.5% fetal bovine serum and 10% horse serum. The ATT20 cell line (ATCC) was cultured in a humidified incubator at 37° C and 5% CO₂ in DMEM (ATCC) supplemented with 10% fetal bovine serum.

The murine pituitary corticotrophin tumor AtT-20 cell line (ATCC CRL-1795) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Rat pituitary tumor GH3 cells and murine RAW 264.7 macrophages were preserved in our laboratory. The AtT-20 and RAW 264.7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco BRL, Grand Island, NY, USA), 100 IU/mL penicillin and 100 μg/mL streptomycin (Solarbio, Beijing, China), and GH3 cells were maintained in Ham’s F-12K medium (Procell, Wuhan, Hubei, China) containing 15% horse serum (Gibco BRL, Grand Island, NY, USA), 2.5% FBS, 100 IU/mL penicillin and 100 μg/mL streptomycin. All cells were cultured at 37 °C in a humidified incubator under 5% CO₂.
GH3 and AtT-20 cells were seeded in 96-well plates at a density of 2 × 10⁴ cells per well and incubated with the drugs at appropriate concentrations for 24 h. The percentage of viable cells was determined using the Cell Counting Kit-8 (KeyGen Biotech, Nanjing, Jiangsu, China) according to the manufacturer’s instructions, and measuring the absorbance at 450 nm. IC₅₀ values were calculated using GraphPad Prism 7 software (GraphPad Software Inc, San Diego, CA, USA).