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2 protocols using rabbit anti tgald polyclonal antibody

1

Immunostaining of Parasite-infected Cells

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Parasite-infected HFF cells were fixed with 4% paraformaldehyde for 15 min and then subjected to immunostaining following previously described protocols (50 (link)). Primary antibodies used include mouse anti-Ty monoclonal antibody, rabbit anti-TgALD polyclonal antibody, rabbit anti-TgIMC1 polyclonal antibody, mouse anti-HA monoclonal antibody (Medical & Biological Laboratories Co., Beijing, China), and rabbit anti-YFP polyclonal antibody (Proteintech, Chicago, USA). Primary antibodies were detected by Alexa-488- or Alexa-594-conjugated secondary antibodies (Life Technologies, CA, USA). Samples were visualized under the Olympus BX53 microscope (Olympus Life Science, Tokyo, Japan) equipped with an AxioCam 503 mono camera (Zeiss, Oberkochen, Germany).
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2

Immunostaining of Parasite-infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Parasite-infected HFF cells were fixed with 4% paraformaldehyde for 15 min and then subjected to immunostaining following previously described protocols (50 (link)). Primary antibodies used include mouse anti-Ty monoclonal antibody, rabbit anti-TgALD polyclonal antibody, rabbit anti-TgIMC1 polyclonal antibody, mouse anti-HA monoclonal antibody (Medical & Biological Laboratories Co., Beijing, China), and rabbit anti-YFP polyclonal antibody (Proteintech, Chicago, USA). Primary antibodies were detected by Alexa-488- or Alexa-594-conjugated secondary antibodies (Life Technologies, CA, USA). Samples were visualized under the Olympus BX53 microscope (Olympus Life Science, Tokyo, Japan) equipped with an AxioCam 503 mono camera (Zeiss, Oberkochen, Germany).
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