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Prescission

Manufactured by Eurofins
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PreScission is a lab equipment product offered by Eurofins. It is designed to perform precise and controlled sample preparation tasks. The core function of PreScission is to facilitate sample processing and handling, though its specific intended use is not provided in this unbiased, factual description.

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Lab products found in correlation

Prescission, by GE Healthcare (2 mentions) Puc57, by GenScript (2 mentions)

2 protocols using prescission

1

Purification of Codon-Optimized CroS Protein

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The gene encoding R391 croS was codon optimized for expression in E. coli and chemically synthesized (Genscript) as a 309 bp NdeI‐PstI fragment and cloned into pUC57 (Genscript). The NdeI‐PstI fragment was subsequently subcloned into the same sites of pCF2. pCF2 is a medium copy plasmid that expresses Isopropyl ß‐D‐1‐thiogalactopyranoside‐inducible glutathione‐S transferase (GST). The construct also contains a PreScission (GE Healthcare) protease site immediately downstream of the GST protein and upstream of the unique NdeI site. When the target gene is cloned into the NdeI site of pCF2, a GST‐fusion protein is generated with a PreScission site immediately upstream of the target protein. CroS was initially purified as a GST‐CroS fusion protein as a custom service by scientists at Eurofins, as previously described (Poulin‐Laprade & Burrus, 2015 (link)), and the GST‐affinity tag subsequently removed after the PreScission protease treatment (Eurofins).
McDonald J.P., Quiros D.R., Vaisman A., Mendez A.R., Reyelt J., Schmidt M., Gonzalez M, & Woodgate R. (2021). CroSR391, an ortholog of the λ Cro repressor, plays a major role in suppressing polVR391‐dependent mutagenesis. Molecular Microbiology, 116(3), 877-889.
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2

Purification of Codon-Optimized CroS Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
The gene encoding R391 croS was codon optimized for expression in E. coli and chemically synthesized (Genscript) as a 309 bp NdeI-PstI fragment and cloned into pUC57 (Genscript). The NdeI-PstI fragment was subsequently sub-cloned into the same sites of pCF2. pCF2 is a medium copy plasmid that expresses IPTG-inducible glutathione-S transferase (GST). The construct also contains a PreScission (GE Healthcare) protease site immediately downstream of the GST protein and upstream of the unique NdeI site. When the target gene is cloned into the NdeI site of pCF2, a GST-fusion protein is generated with a PreScission site immediately upstream of the target protein. CroS was initially purified as a GST-CroS fusion protein as a custom service by scientists at Eurofins, as previously described (Poulin-Laprade & Burrus, 2015 (link)) and the GST-affinity tag subsequently removed after PreScission protease treatment (Eurofins).
McDonald J.P., Quiros D.R., Vaisman A., Mendez A.R., Reyelt J., Schmidt M., Gonzalez M, & Woodgate R. (2021). CroSR391, an ortholog of the λ Cro repressor, plays a major role in suppressing polVR391‐dependent mutagenesis. Molecular Microbiology, 116(3), 877-889.
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