Log In Sign up
The largest database of trusted experimental protocols

Fitc anti human cd123

Manufactured by BioLegend
Visit Supplier

FITC anti-human CD123 is a fluorochrome-conjugated monoclonal antibody that specifically binds to the human CD123 protein, also known as the Interleukin-3 receptor alpha chain. It is designed for use in flow cytometry applications to identify and quantify CD123-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Stain buffer, by BD (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Percp cy 5.5 mouse anti human hla dr, by BD (1 mentions) Fitc anti human cd14, by BioLegend (1 mentions) Fitc anti human cd34, by BioLegend (1 mentions) Alexa fluor 700 anti human cd3, by BioLegend (1 mentions) Apc anti human cd336 nkp44, by BioLegend (1 mentions) Fitc anti human tcrαβ, by BioLegend (1 mentions) Fitc anti human tcrγδ, by BioLegend (1 mentions) Alexa fluor 488 anti human cd19, by BioLegend (1 mentions) Fitc anti human fcεriα, by BioLegend (1 mentions)

Spelling variants of this product

CD123‐FITC (3 citations) Anti-CD123-FITC (2 citations)

2 protocols using fitc anti human cd123

1

Quantification of Plasmacytoid Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples were collected at 0, 6, and 12 weeks after the start of intake. PBMCs were obtained from blood samples, frozen, and stored until measurement. The frozen PBMCs were thawed, and live cells were counted by trypan blue staining. A total of 5 × 105 live cells were suspended with Stain Buffer (BD) and treated with BD Fc Block™ Reagent for Human (BD) at 4 °C for 10 min. Subsequently, fluorescent dye-conjugated antibodies (FITC anti-human CD123, APC anti-human CD304 [Neuropilin-1; BioLegend], PE anti-human CD86 [Thermo Fisher Scientific], PerCP-Cy™5.5 mouse anti-human HLA-DR [BD]) were added and the cells were stained at 4 °C for 30 min in the dark. Stained cells were fixed with 4% paraformaldehyde/PBS and measured using Accuri C6 flow cytometer (BD). CD123+ CD304+ cells were gated as pDCs, and the expression levels of cell surface markers (CD86 and HLA-DR) were used to measure pDC activity.
Kobatake E., Iwama Y., Arai T., Tsukisaka Y, & Kabuki T. (2023). Lactobacillus paragasseri SBT2055 Activates Plasmacytoid Dendritic Cells and Improves Subjective Symptoms of Common Cold in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Parallel-Group Comparative Trial. Nutrients, 15(20), 4458.
+ Open protocol
+ Expand
2

Multiparametric Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alexa Fluor® 700 anti-human CD3, (clone: HIT3a), FITC-anti human TCRαβ (clone: IP26), FITC-anti-human TCRγδ (clone: B1), APC/Cy7 Anti-Human CD127 (I-7Ra), (clone: A019D5), PE anti-human CD161, (clone: HP-3G10), Brilliant Violet 421TM anti-human CD117 (c-kit), (clone 104D2), PE/Cy7 anti-human CD294 (CRTH2), (clone: BM16), APC anti-human CD336 (NKp44), (clone: 325110), Alexa Fluor® 488 anti-human CD19, (clone: HIB19), FITC anti-human CD94, (clone:DX22), FITC anti-human CD1a, (clone: HI149), FITC anti-human CD11c, (clone: 3.9), FITC anti-human CD123, (clone: 6H6), anti-human CD303 (BDCA-2), (clone:201A), FITC anti-human CD14, (clone: 63D3), FITC anti-human FcεRIα, (clone: NP4D6), FITC anti-human CD34,(clone: 561), APC/Cy7 anti-human IFN-γ, (clone: 4S.B3) all from BioLegend. Anti-Human CD363 (S1PR1) eFluor® 660, (clone: SW4GYPP, ThermoFisher), Mouse IgG1 K Isotype Control eFluor® 660, (clone: P3.6.2.8.1, ThermoFisher). Anti-mouse CD3) APC, (clone: 17A2), anti-mouse NK1.1 Alexa Fluor® 647, (clone: PK136), anti-mouse B220 APC/Cy7 or FITC, (clone:RA3-6B2), anti-mouse CD45-Percp Cy5.5, (clone:30-F11), anti-mouse CD90.2 PE Cy7, (clone:30-H12), anti-mouse CD11b APC, (clone:M1/70), anti-mouse GATA3 PE (Clone: 16E10A23), anti-mouse Rorγt (Clone: 2F7-2).
Eken A., Yetkin M.F., Vural A., Okus F.Z., Erdem S., Azizoglu Z.B., Haliloglu Y., Cakir M., Turkoglu E.M., Kilic O., Kara I., Dönmez Altuntaş H., Oukka M., Kutuk M.S., Mirza M, & Canatan H. (2019). Fingolimod Alters Tissue Distribution and Cytokine Production of Human and Murine Innate Lymphoid Cells. Frontiers in Immunology, 10, 217.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!