Anti cd1a

The following monoclonal antibodies (mAb) were purchased from BioLegend (San Diego, CA) and used for a flow cytometric analysis: purified anti-CD8 (clone 93), Alexa Fluor 647, anti-CD3, anti-CD1a, CD62L, CD44, CD127 and anti-CD16/CD32 FITC-labeled anti-CD8 (clone 53-6.7), PE-Cy7-labeled anti-CD3 (clone 17A2), PerCP-Cy5.5-labeled anti-CD62L (clone MEL-14), PE-labeled anti-CD11a (clone 2D7) and APC-Cy7-labeled anti-CD44 (clone IM7).

Cell phenotype staining was performed using the following antibodies: mouse anti-human MGL (HML clone, MoAb MLD-1 [25 (link)]; a generous gift from Professor Tatsura Irimura), mouse anti-human MUC1 (HMFG2), mouse anti-STn (TKH2,) followed by FITC-conjugated goat anti-mouse IgG (H+L; DAKO). MoAbs directly conjugated with FITC or phycoerythrin were also used (all from Beckman Coulter unless stated): IgG₁-PE or FITC as isotype controls, anti-MGL (Biolegend; clone H037G3), anti-CD1a, anti-CD83, anti-CD80, anti-CD86 (all PE), anti-CD14, anti-HLA-DR, anti-CCR7, anti-CD40 (all FITC). Cells were suspended in PBS + 0.5% BSA (2 × 10⁵ cells/100 μL/sample) and incubated with MoAbs according to the manufacturer's instructions. At least 1 × 10⁴ events were evaluated using either Epics XL, (Beckman Coulter) or FACSCalibur (BD Biosciences) flow cytometers. Analysis was performed using either WinMDI or Cellquest software.