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P s6 d57.2.2e

Manufactured by Cell Signaling Technology
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P-S6 (D57.2.2E) is a laboratory reagent produced by Cell Signaling Technology. It is an antibody that detects phosphorylated S6 ribosomal protein. The core function of this product is to facilitate the detection and analysis of phosphorylated S6 in biological samples.

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Lab products found in correlation

Celltrace violet, by Thermo Fisher Scientific (2 mentions) Monensin, by Thermo Fisher Scientific (2 mentions) Anti cd71 r17217, by Thermo Fisher Scientific (2 mentions) Anti cd98 rl388, by Thermo Fisher Scientific (2 mentions) Active caspase 3 apoptosis kit, by BD (2 mentions) C myc d84c12, by Cell Signaling Technology (2 mentions) Bim c34c5, by Cell Signaling Technology (2 mentions) Lsrfortessa, by BD (2 mentions) Fix perm buffer, by BD (1 mentions) Perm wash buffer, by BD (1 mentions)

Close spelling variants of this product

Anti-p-S6(S235/236) (D57.2.2E) P-Ser235.236-S6 ribosomal protein (D57.2.2E,

3 protocols using p s6 d57.2.2e

1

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of surface markers, cells were stained in PBS containing 2% (wt/vol) BSA, with anti-CD4 (RM4-5), anti-CD8α (53-6.7), anti-TCRβ (H57-597), anti-CD44 (1M7), anti-CD62L (MEL-14), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD71 (R17217), and anti-CD98 (RL388; all from eBioscience). Intracellular Foxp3 (FJK-16s), Ki67 (SolA15), IFN-γ (XMG1.2), IL-4 (11B11), IL-17 (17B7; all from eBioscience), Bim (C34C5), c-Myc (D84C12), and p-S6 (D57.2.2E; all from Cell Signaling Technology) were analyzed by flow cytometry according to the manufacturer’s instructions. For intracellular cytokine staining, T cells were stimulated for 4 h with PMA plus ionomycin in the presence of monensin before intracellular staining according to the manufacturer’s instructions (eBioscience). Caspase-3 activity was measured using active caspase-3 apoptosis kit (BD Biosciences). To monitor cell division, lymphocytes were labeled with CellTrace™ violet (Life Technologies). Flow cytometry data were acquired on LSRII or LSR Fortessa (BD Biosciences) and analyzed using Flowjo software (Tree Star).
Wei J., Long L., Yang K., Guy C., Shrestha S., Chen Z., Wu C., Vogel P., Neale G., Green D.R, & Chi H. (2016). Autophagy enforces functional integrity of regulatory T cells by coupling environmental cues and metabolic homeostasis. Nature immunology, 17(3), 277-285.
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2

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of surface markers, cells were stained in PBS containing 2% (wt/vol) BSA, with anti-CD4 (RM4-5), anti-CD8α (53-6.7), anti-TCRβ (H57-597), anti-CD44 (1M7), anti-CD62L (MEL-14), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD71 (R17217), and anti-CD98 (RL388; all from eBioscience). Intracellular Foxp3 (FJK-16s), Ki67 (SolA15), IFN-γ (XMG1.2), IL-4 (11B11), IL-17 (17B7; all from eBioscience), Bim (C34C5), c-Myc (D84C12), and p-S6 (D57.2.2E; all from Cell Signaling Technology) were analyzed by flow cytometry according to the manufacturer’s instructions. For intracellular cytokine staining, T cells were stimulated for 4 h with PMA plus ionomycin in the presence of monensin before intracellular staining according to the manufacturer’s instructions (eBioscience). Caspase-3 activity was measured using active caspase-3 apoptosis kit (BD Biosciences). To monitor cell division, lymphocytes were labeled with CellTrace™ violet (Life Technologies). Flow cytometry data were acquired on LSRII or LSR Fortessa (BD Biosciences) and analyzed using Flowjo software (Tree Star).
Wei J., Long L., Yang K., Guy C., Shrestha S., Chen Z., Wu C., Vogel P., Neale G., Green D.R, & Chi H. (2016). Autophagy enforces functional integrity of regulatory T cells by coupling environmental cues and metabolic homeostasis. Nature immunology, 17(3), 277-285.
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3

Intracellular Antigen Staining Protocol

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Approximately one fourth of each spleen was immediately disrupted between frosted glass slides into BD Fix/Perm buffer (BD Biosciences). Cells were incubated for 20 min at RT, washed in FACS buffer, and resuspended in 90% ice-cold methanol for ≥30 min. Cells were washed with BD Perm/Wash buffer (BD Biosciences) and stained for surface and intracellular antigens, including p-S6 (D57.2.2E; Cell Signaling Technology), for 45 min at RT.
Mittelsteadt K.L., Hayes E.T, & Campbell D.J. (2021). ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue. The Journal of Experimental Medicine, 218(6), e20201142.
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